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Cytoskeletal components can turn wall-less spherical bacteria into kinking helices

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLARTIGUE, Carole
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLAMBERT, Bastien
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorRIDEAU, Fabien
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDAHAN, Yorick
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDECOSSAS, Marion
hal.structure.identifierUniversité de Rouen Normandie [UNIROUEN]
dc.contributor.authorHILLION, Mélanie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDOULIEZ, Jean-Paul
hal.structure.identifierUniversité de Rouen Normandie [UNIROUEN]
dc.contributor.authorHARDOUIN, Julie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLAMBERT, Olivier
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBLANCHARD, Alain
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBÉVEN, Laure
dc.date.issued2022-11-14
dc.identifier.issn2041-1723
dc.description.abstractEnBacterial cell shape is generally determined through an interplay between the peptidoglycan cell wall and cytoplasmic filaments made of polymerized MreB. Indeed, some bacteria (e.g., Mycoplasma ) that lack both a cell wall and mreB genes consist of non-motile cells that are spherical or pleomorphic. However, other members of the same class Mollicutes (e.g., Spiroplasma , also lacking a cell wall) display a helical cell shape and kink-based motility, which is thought to rely on the presence of five MreB isoforms and a specific fibril protein. Here, we show that heterologous expression of Spiroplasma fibril and MreB proteins confers helical shape and kinking ability to Mycoplasma capricolum cells. Isoform MreB5 is sufficient to confer helicity and kink propagation to mycoplasma cells. Cryoelectron microscopy confirms the association of cytoplasmic MreB filaments with the plasma membrane, suggesting a direct effect on membrane curvature. However, in our experiments, the heterologous expression of MreBs and fibril did not result in efficient motility in culture broth, indicating that additional, unknown Spiroplasma components are required for swimming.
dc.description.sponsorshipTransition Sphère-Hélice de la forme de cellules minimales sans paroi - ANR-22-CE44-0015
dc.language.isoen
dc.publisherNature Publishing Group
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.title.enCytoskeletal components can turn wall-less spherical bacteria into kinking helices
dc.typeArticle de revue
dc.identifier.doi10.1038/s41467-022-34478-0
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalNature Communications
bordeaux.page6930
bordeaux.volume13
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-03914264
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03914264v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature%20Communications&rft.date=2022-11-14&rft.volume=13&rft.issue=1&rft.spage=6930&rft.epage=6930&rft.eissn=2041-1723&rft.issn=2041-1723&rft.au=LARTIGUE,%20Carole&LAMBERT,%20Bastien&RIDEAU,%20Fabien&DAHAN,%20Yorick&DECOSSAS,%20Marion&rft.genre=article


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