LARTIGUE, Carole; LAMBERT, Bastien; RIDEAU, Fabien; DAHAN, Yorick; DECOSSAS, Marion; HILLION, Mélanie; DOULIEZ, Jean-Paul; HARDOUIN, Julie; LAMBERT, Olivier; BLANCHARD, Alain; BÉVEN, Laure

doi:10.1038/s41467-022-34478-0

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LARTIGUE, Carole
Biologie du fruit et pathologie [BFP]

LAMBERT, Bastien
Biologie du fruit et pathologie [BFP]

RIDEAU, Fabien
Biologie du fruit et pathologie [BFP]

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Article de revue

Ce document a été publié dans

Nature Communications. 2022-11-14, vol. 13, n° 1, p. 6930

Nature Publishing Group

Résumé en anglais

Bacterial cell shape is generally determined through an interplay between the peptidoglycan cell wall and cytoplasmic filaments made of polymerized MreB. Indeed, some bacteria (e.g., Mycoplasma ) that lack both a cell wall and mreB genes consist of non-motile cells that are spherical or pleomorphic. However, other members of the same class Mollicutes (e.g., Spiroplasma , also lacking a cell wall) display a helical cell shape and kink-based motility, which is thought to rely on the presence of five MreB isoforms and a specific fibril protein. Here, we show that heterologous expression of Spiroplasma fibril and MreB proteins confers helical shape and kinking ability to Mycoplasma capricolum cells. Isoform MreB5 is sufficient to confer helicity and kink propagation to mycoplasma cells. Cryoelectron microscopy confirms the association of cytoplasmic MreB filaments with the plasma membrane, suggesting a direct effect on membrane curvature. However, in our experiments, the heterologous expression of MreBs and fibril did not result in efficient motility in culture broth, indicating that additional, unknown Spiroplasma components are required for swimming.< Réduire

DOI

http://dx.doi.org/10.1038/s41467-022-34478-0

Project ANR

Transition Sphère-Hélice de la forme de cellules minimales sans paroi - ANR-22-CE44-0015

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Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332