Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq
| dc.rights.license | open | en_US |
| dc.contributor.author | TURBANT, Florian | |
| dc.contributor.author | WAEYTENS, Jehan | |
| dc.contributor.author | CAMPIDELLI, Camille | |
| dc.contributor.author | BOMBLED, Marianne | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | MARTINEZ, Denis | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | GRELARD, Axelle | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | HABENSTEIN, Birgit | |
| dc.contributor.author | RAUSSENS, Vincent | |
| dc.contributor.author | VELEZ, Marisela | |
| dc.contributor.author | WIEN, Frank | |
| dc.contributor.author | ARLUISON, Véronique | |
| dc.date.accessioned | 2023-04-26T12:47:02Z | |
| dc.date.available | 2023-04-26T12:47:02Z | |
| dc.date.issued | 2022-08-05 | |
| dc.identifier.issn | 1422-0067 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/173215 | |
| dc.description.abstractEn | Hfq is a pleiotropic regulator that mediates several aspects of bacterial RNA metabolism. The protein notably regulates translation efficiency and RNA decay in Gram-negative bacteria, usually via its interaction with small regulatory RNAs. Previously, we showed that the Hfq C-terminal region forms an amyloid-like structure and that these fibrils interact with membranes. The immediate consequence of this interaction is a disruption of the membrane, but the effect on Hfq structure was unknown. To investigate details of the mechanism of interaction, the present work uses different in vitro biophysical approaches. We show that the Hfq C-terminal region influences membrane integrity and, conversely, that the membrane specifically affects the amyloid assembly. The reported effect of this bacterial master regulator on membrane integrity is discussed in light of the possible consequence on small regulatory RNA-based regulation. | |
| dc.description.sponsorship | Université de Paris - ANR-18-IDEX-0001 | en_US |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject.en | Small non-coding RNA | |
| dc.subject.en | Regulatory RNA | |
| dc.subject.en | Hfq | |
| dc.subject.en | Bacterial amyloid | |
| dc.subject.en | Protein self-assembly | |
| dc.subject.en | Membrane insertion | |
| dc.subject.en | Lipid–protein interaction | |
| dc.title.en | Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.3390/ijms23158739 | en_US |
| dc.subject.hal | Chimie/Matériaux | en_US |
| bordeaux.journal | International Journal of Molecular Sciences | en_US |
| bordeaux.volume | 23 | en_US |
| bordeaux.hal.laboratories | CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248 | en_US |
| bordeaux.issue | 15 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | Bordeaux INP | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.identifier | hal-04082530 | |
| hal.version | 1 | |
| hal.date.transferred | 2023-04-26T12:47:07Z | |
| hal.export | true | |
| dc.rights.cc | CC BY | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=International%20Journal%20of%20Molecular%20Sciences&rft.date=2022-08-05&rft.volume=23&rft.issue=15&rft.eissn=1422-0067&rft.issn=1422-0067&rft.au=TURBANT,%20Florian&WAEYTENS,%20Jehan&CAMPIDELLI,%20Camille&BOMBLED,%20Marianne&MARTINEZ,%20Denis&rft.genre=article |
