TURBANT, Florian; WAEYTENS, Jehan; CAMPIDELLI, Camille; BOMBLED, Marianne; MARTINEZ, Denis; GRELARD, Axelle; HABENSTEIN, Birgit; RAUSSENS, Vincent; VELEZ, Marisela; WIEN, Frank; ARLUISON, Véronique

doi:10.3390/ijms23158739

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Article de revue

Ce document a été publié dans

International Journal of Molecular Sciences. 2022-08-05, vol. 23, n° 15

Résumé en anglais

Hfq is a pleiotropic regulator that mediates several aspects of bacterial RNA metabolism. The protein notably regulates translation efficiency and RNA decay in Gram-negative bacteria, usually via its interaction with small regulatory RNAs. Previously, we showed that the Hfq C-terminal region forms an amyloid-like structure and that these fibrils interact with membranes. The immediate consequence of this interaction is a disruption of the membrane, but the effect on Hfq structure was unknown. To investigate details of the mechanism of interaction, the present work uses different in vitro biophysical approaches. We show that the Hfq C-terminal region influences membrane integrity and, conversely, that the membrane specifically affects the amyloid assembly. The reported effect of this bacterial master regulator on membrane integrity is discussed in light of the possible consequence on small regulatory RNA-based regulation.< Réduire

Mots clés en anglais

Small non-coding RNA

Regulatory RNA

Hfq

Bacterial amyloid

Protein self-assembly

Membrane insertion

Lipid–protein interaction

URI

https://oskar-bordeaux.fr/handle/20.500.12278/173215

DOI

http://dx.doi.org/10.3390/ijms23158739

Project ANR

Université de Paris - ANR-18-IDEX-0001

Unités de recherche

CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248

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Unraveling Membrane Perturbations Caused by the Bacterial Riboregulator Hfq