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Cell-assembled extracellular matrix (CAM): a human biopaper for the biofabrication of pre-vascularized tissues able to connect to the host circulation in vivo

dc.rights.licenseopenen_US
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorOLIVEIRA, Hugo
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorMEDINA, Chantal
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorLABRUNIE, G.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorDUSSERRE, Nathalie
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorCATROS, Sylvain
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorMAGNAN, L.
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorHANDSCHIN, Charles
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorSTACHOWICZ, Marie-Laure
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorFRICAIN, Jean-Christophe
ORCID: 0000-0001-7855-6437
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorL'HEUREUX, Nicolas
dc.date.accessioned2023-04-04T14:16:01Z
dc.date.available2023-04-04T14:16:01Z
dc.date.issued2021-10
dc.identifier.issn1758-5090en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/172737
dc.description.abstractEnWhen considering regenerative approaches, the efficient creation of a functional vasculature, that can support the metabolic needs of bioengineered tissues, is essential for their survival after implantation. However, it is widely recognized that the post-implantation microenvironment of the engineered tissues is often hypoxic due to insufficient vascularization, resulting in ischemia injury and necrosis. This is one of the main limitations of current tissue engineering applications aiming at replacing significant tissue volumes. Here, we have explored the use of a new biomaterial, the cell-assembled extracellular matrix (CAM), as a biopaper to biofabricate a vascular system. CAM sheets are a unique, fully biological and fully human material that has already shown stable long-term implantation in humans. We demonstrated, for the first time, the use of this unprocessed human ECM as a microperforated biopaper. Using microvalve dispensing bioprinting, concentrated human endothelial cells (30 millions ml−1) were deposited in a controlled geometry in CAM sheets and cocultured with HSFs. Following multilayer assembly, thick ECM-based constructs fused and supported the survival and maturation of capillary-like structures for up to 26 d of culture. Following 3 weeks of subcutaneous implantation in a mice model, constructs showed limited degradative response and the pre-formed vasculature successfully connected with the host circulatory system to establish active perfusion.This mechanically resilient tissue equivalent has great potential for the creation of more complex implantable tissues, where rapid anastomosis is sine qua non for cell survival and efficient tissue integration.
dc.language.isoENen_US
dc.subject.encell-assembled extracellular matrix
dc.subject.enbioprinting
dc.subject.enlaser microdissection
dc.subject.envasculogenesis
dc.title.enCell-assembled extracellular matrix (CAM): a human biopaper for the biofabrication of pre-vascularized tissues able to connect to the host circulation in vivo
dc.title.alternativeBiofabricationen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1088/1758-5090/ac2f81en_US
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologieen_US
bordeaux.journalBiofabricationen_US
bordeaux.volume14en_US
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026en_US
bordeaux.issue1en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.institutionINSERMen_US
bordeaux.institutionCHU de Bordeauxen_US
bordeaux.institutionInstitut Bergoniéen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-04058210
hal.version1
hal.date.transferred2023-04-04T14:16:07Z
hal.exporttrue
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biofabrication&rft.date=2021-10&rft.volume=14&rft.issue=1&rft.eissn=1758-5090&rft.issn=1758-5090&rft.au=OLIVEIRA,%20Hugo&MEDINA,%20Chantal&LABRUNIE,%20G.&DUSSERRE,%20Nathalie&CATROS,%20Sylvain&rft.genre=article


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