Show simple item record

dc.rights.licenseopenen_US
dc.contributor.authorGUILBAULT, Samuel
dc.contributor.authorGARRIGUE, Patrick
dc.contributor.authorGARNIER, Leo
dc.contributor.authorPANDARD, Justine
dc.contributor.authorLEMAITRE, Frederic
dc.contributor.authorGUILLE-COLLIGNON, Manon
dc.contributor.authorSOJIC, Neso
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorARBAULT, Stephane
dc.date.accessioned2023-04-03T09:40:17Z
dc.date.available2023-04-03T09:40:17Z
dc.date.issued2022-12-01
dc.identifier.issn1567-5394en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/172677
dc.description.abstractEnOptical fibers have opened avenues for remote imaging, bioanalyses and recently optogenetics. Besides, miniaturized electrochemical sensors have offered new opportunities in sensing directly redox neurotransmitters. The combination of both optical and electrochemical approaches was usually performed on the platform of microscopes or within microsystems. In this work, we developed optoelectrodes which features merge the advantages of both optical fibers and microelectrodes. Optical fiber bundles were modified at one of their extremity by a transparent ITO deposit. The electrochemical responses of these ITO-modified bundles were characterized for the detection of dopamine, epinephrine and norepinephrine. The analytical performances of the optoelectrodes were equivalent to the ones reported for carbon microelectrodes. The remote imaging of model neurosecretory PC12 cells by optoelectrodes was performed upon cell-staining with common fluorescent dyes: acridine orange and calcein-AM. An optoelectrode placed by micromanipulation at a few micrometers-distance from the cells offered remote images with single cell resolution. Finally, in situ electrochemical sensing was demonstrated by additions of K+-secretagogue solutions near PC12 cells under observation, leading to exocytotic events detected as amperometric spikes at the ITO surface. Such dual sensors should pave the way for in vivo remote imaging, optogenetic stimulation, and simultaneous detection of neurosecretory activities.
dc.language.isoENen_US
dc.subject.enOptoelectrode
dc.subject.enElectrochemistry
dc.subject.enCatecholamine
dc.subject.enITO
dc.subject.enImaging
dc.subject.enOptogenetics
dc.title.enDesign of optoelectrodes for the remote imaging of cells and in situ electrochemical detection of neurosecretory events
dc.title.alternativeBioelectrochemistryen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.bioelechem.2022.108262en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalBioelectrochemistryen_US
bordeaux.volume148en_US
bordeaux.hal.laboratoriesCBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.exportfalse
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Bioelectrochemistry&rft.date=2022-12-01&rft.volume=148&rft.eissn=1567-5394&rft.issn=1567-5394&rft.au=GUILBAULT,%20Samuel&GARRIGUE,%20Patrick&GARNIER,%20Leo&PANDARD,%20Justine&LEMAITRE,%20Frederic&rft.genre=article


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record