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Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells
dc.rights.license | open | en_US |
hal.structure.identifier | Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV] | |
dc.contributor.author | KONG, Junhua | |
hal.structure.identifier | Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV] | |
dc.contributor.author | GARCIA, Virginie | |
hal.structure.identifier | Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV] | |
dc.contributor.author | ZEHRAOUI, Enric | |
hal.structure.identifier | Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV] | |
dc.contributor.author | STAMMITTI, Linda | |
hal.structure.identifier | Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV] | |
dc.contributor.author | GODINAUD, Ghislaine
IDREF: 225640449 | |
hal.structure.identifier | Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV] | |
dc.contributor.author | RENAUD, Christel | |
dc.contributor.author | MAURY, Stéphane | |
dc.contributor.author | DELAUNAY, Alain | |
hal.structure.identifier | Unité de Recherche Œnologie [Villenave d'Ornon] [OENO] | |
dc.contributor.author | CLUZET, Stéphanie | |
hal.structure.identifier | Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV] | |
dc.contributor.author | LECOURIEUX, Fatma
IDREF: 158692306 | |
hal.structure.identifier | Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV] | |
dc.contributor.author | LECOURIEUX, David
IDREF: 061722464 | |
hal.structure.identifier | Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV] | |
dc.contributor.author | TEYSSIER, Emeline | |
hal.structure.identifier | Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV] | |
dc.contributor.author | GALLUSCI, Philippe | |
dc.date.accessioned | 2023-02-20T16:50:22Z | |
dc.date.available | 2023-02-20T16:50:22Z | |
dc.date.issued | 2022-07-15 | |
dc.identifier.issn | 2073-4425 | en_US |
dc.identifier.other | https://mdpi-res.com/d_attachment/genes/genes-13-01256/article_deploy/genes-13-01256-s001.zip?version=1657884457 | en_US |
dc.identifier.other | https://www.ebi.ac.uk/ena/browser/view/PRJEB54585 | en_US |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/172010 | |
dc.description.abstractEn | Through its role in the regulation of gene expression, DNA methylation can participate in the control of specialized metabolite production. We have investigated the link between DNA methylation and anthocyanin accumulation in grapevine using the hypomethylating drug, zebularine and Gamay Teinturier cell suspensions. In this model, zebularine increased anthocyanin accumulation in the light, and induced its production in the dark. To unravel the underlying mechanisms, cell transcriptome, metabolic content, and DNA methylation were analyzed. The up-regulation of stress-related genes, as well as a decrease in cell viability, revealed that zebularine affected cell integrity. Concomitantly, the global DNA methylation level was only slightly decreased in the light and not modified in the dark. However, locus-specific analyses demonstrated a decrease in DNA methylation at a few selected loci, including a CACTA DNA transposon and a small region upstream from the UFGT gene, coding for the UDP glucose:flavonoid-3-O-glucosyltransferase, known to be critical for anthocyanin biosynthesis. Moreover, this decrease was correlated with an increase in UFGT expression and in anthocyanin content. In conclusion, our data suggest that UFGT expression could be regulated through DNA methylation in Gamay Teinturier, although the functional link between changes in DNA methylation and UFGT transcription still needs to be demonstrated. | |
dc.language.iso | EN | en_US |
dc.rights | Attribution 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
dc.subject.en | DNA methylation | |
dc.subject.en | Zebularine | |
dc.subject.en | Anthocyanin | |
dc.title.en | Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells | |
dc.type | Article de revue | en_US |
dc.identifier.doi | 10.3390/genes13071256 | en_US |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale | en_US |
bordeaux.journal | Genes | en_US |
bordeaux.page | 1256 | en_US |
bordeaux.volume | 13 | en_US |
bordeaux.hal.laboratories | Ecophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287 | en_US |
bordeaux.issue | 7 | en_US |
bordeaux.institution | Université de Bordeaux | en_US |
bordeaux.institution | Bordeaux Sciences Agro | en_US |
bordeaux.institution | INRAE | en_US |
bordeaux.peerReviewed | oui | en_US |
bordeaux.inpress | non | en_US |
hal.export | false | |
dc.rights.cc | CC BY | en_US |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Genes&rft.date=2022-07-15&rft.volume=13&rft.issue=7&rft.spage=1256&rft.epage=1256&rft.eissn=2073-4425&rft.issn=2073-4425&rft.au=KONG,%20Junhua&GARCIA,%20Virginie&ZEHRAOUI,%20Enric&STAMMITTI,%20Linda&GODINAUD,%20Ghislaine&rft.genre=article |