Determination of Interactive States of Immune Checkpoint Regulators in Lung Metastases after Radiofrequency Ablation
| dc.rights.license | open | en_US |
| dc.contributor.author | MILES, James | |
| dc.contributor.author | SOUBEYRAN, Isabelle | |
| dc.contributor.author | MARLIOT, Florence | |
| dc.contributor.author | PANGON, Nicolas | |
| dc.contributor.author | ITALIANO, Antoine | |
| hal.structure.identifier | Bordeaux population health [BPH] | |
| dc.contributor.author | BELLERA, Carine | |
| dc.contributor.author | WARD, Stephen G. | |
| dc.contributor.author | PAGES, Franck | |
| dc.contributor.author | PALUSSIERE, Jean | |
| dc.contributor.author | LARIJANI, Banafshe | |
| dc.date.accessioned | 2023-02-17T10:21:22Z | |
| dc.date.available | 2023-02-17T10:21:22Z | |
| dc.date.issued | 2022-11-22 | |
| dc.identifier.issn | 2072-6694 (Print) 2072-6694 (Electronic) 2072-6694 (Linking) | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/171982 | |
| dc.description.abstractEn | BACKGROUND: Cases of the spontaneous regression of multiple pulmonary metastases, after radiofrequency ablation (RFA), of a single lung metastasis, have been documented to be mediated by the immune system. The interaction of immune checkpoints, e.g., PD-1/PD-L1 and CTLA-4/CD80, may explain this phenomenon. The purpose of this study is to identify and quantify immune mechanisms triggered by RFA of pulmonary metastases originating from colorectal cancer. METHODS: We used two-site time-resolved Förster resonance energy transfer as determined by frequency-domain FLIM (iFRET) for the quantification of receptor-ligand interactions. iFRET provides a method by which immune checkpoint interaction states can be quantified in a spatiotemporal manner. The same patient sections were used for assessment of ligand-receptor interaction and intratumoral T-cell labeling. CONCLUSION: The checkpoint interaction states quantified by iFRET did not correlate with ligand expression. We show that immune checkpoint ligand expression as a predictive biomarker may be unsuitable as it does not confirm checkpoint interactions. In pre-RFA-treated metastases, there was a significant and negative correlation between PD-1/PD-L1 interaction state and intratumoral CD3+ and CD8+ density. The negative correlation of CD8+ and interactive states of PD-1/PD-L1 can be used to assess the state of immune suppression in RFA-treated patients. | |
| dc.language.iso | EN | en_US |
| dc.rights | Attribution 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
| dc.subject.en | Immune checkpoints | |
| dc.subject.en | PD-1/PD-L1 | |
| dc.subject.en | CTLA-4/CD80 | |
| dc.subject.en | Radiofrequency ablation | |
| dc.subject.en | Abscopal effect | |
| dc.subject.en | FRET/FLIM | |
| dc.subject.en | Immune surveyance | |
| dc.title.en | Determination of Interactive States of Immune Checkpoint Regulators in Lung Metastases after Radiofrequency Ablation | |
| dc.title.alternative | Cancers (Basel) | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.3390/cancers14235738 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Santé publique et épidémiologie | en_US |
| dc.identifier.pubmed | 36497220 | en_US |
| bordeaux.journal | Cancers | en_US |
| bordeaux.volume | 14 | en_US |
| bordeaux.hal.laboratories | Bordeaux Population Health Research Center (BPH) - UMR 1219 | en_US |
| bordeaux.issue | 23 | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.institution | INSERM | en_US |
| bordeaux.team | EPICENE_BPH | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.identifier | hal-03993898 | |
| hal.version | 1 | |
| hal.date.transferred | 2023-02-17T10:21:26Z | |
| hal.export | true | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cancers&rft.date=2022-11-22&rft.volume=14&rft.issue=23&rft.eissn=2072-6694%20(Print)%202072-6694%20(Electronic)%202072-6694%20(Linking)&rft.issn=2072-6694%20(Print)%202072-6694%20(Electronic)%202072-6694%20(Linking)&rft.au=MILES,%20James&SOUBEYRAN,%20Isabelle&MARLIOT,%20Florence&PANGON,%20Nicolas&ITALIANO,%20Antoine&rft.genre=article |
