Active image optimization for lattice light sheet microscopy in thick samples
dc.rights.license | open | en_US |
hal.structure.identifier | Bordeaux Imaging Center [BIC] | |
dc.contributor.author | MALIVERT, Maxime | |
dc.contributor.author | HARMS, Fabrice | |
dc.contributor.author | VEILLY, Cynthia | |
dc.contributor.author | LEGRAND, Jerome | |
hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
dc.contributor.author | LI, Ziqiang | |
hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
dc.contributor.author | BAYER, Emmanuelle | |
hal.structure.identifier | Bordeaux Imaging Center [BIC] | |
dc.contributor.author | CHOQUET, Daniel | |
hal.structure.identifier | Bordeaux Imaging Center [BIC] | |
dc.contributor.author | DUCROS, Mathieu | |
dc.date.accessioned | 2023-01-26T09:26:38Z | |
dc.date.available | 2023-01-26T09:26:38Z | |
dc.date.issued | 2022-12-01 | |
dc.identifier.issn | 2156-7085 | en_US |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/171799 | |
dc.description.abstractEn | Lattice light-sheet microscopy (LLSM) is a very efficient technique for high resolution 3D imaging of dynamic phenomena in living biological samples. However, LLSM imaging remains limited in depth due to optical aberrations caused by sample-based refractive index mismatch. Here, we propose a simple and low-cost active image optimization (AIO) method to recover high resolution imaging inside thick biological samples. AIO is based on (1) a light-sheet autofocus step (AF) followed by (2) an adaptive optics image-based optimization. We determine the optimum AIO parameters to provide a fast, precise and robust aberration correction on biological samples. Finally, we demonstrate the performances of our approach on sub-micrometric structures in brain slices and plant roots. | |
dc.language.iso | EN | en_US |
dc.title.en | Active image optimization for lattice light sheet microscopy in thick samples | |
dc.title.alternative | Biomed. Opt. Express, BOE | en_US |
dc.type | Article de revue | en_US |
dc.identifier.doi | 10.1364/BOE.471757 | en_US |
dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire | en_US |
dc.description.sponsorshipEurope | The function of membrane tethering in plant intercellular communication | en_US |
dc.description.sponsorshipEurope | AMPA Receptor Dynamic Organization and Synaptic transmission in health and disease | en_US |
bordeaux.journal | Biomedical optics express | en_US |
bordeaux.page | 6211-6228 | en_US |
bordeaux.volume | 13 | en_US |
bordeaux.hal.laboratories | Laboratoire de Biogenèse Membranaire (LBM) - UMR 5200 | en_US |
bordeaux.issue | 12 | en_US |
bordeaux.institution | Université de Bordeaux | en_US |
bordeaux.institution | CNRS | en_US |
bordeaux.peerReviewed | oui | en_US |
bordeaux.inpress | non | en_US |
hal.identifier | hal-03957311 | |
hal.version | 1 | |
hal.date.transferred | 2023-01-26T09:26:49Z | |
hal.export | true | |
dc.rights.cc | Pas de Licence CC | en_US |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomedical%20optics%20express&rft.date=2022-12-01&rft.volume=13&rft.issue=12&rft.spage=6211-6228&rft.epage=6211-6228&rft.eissn=2156-7085&rft.issn=2156-7085&rft.au=MALIVERT,%20Maxime&HARMS,%20Fabrice&VEILLY,%20Cynthia&LEGRAND,%20Jerome&LI,%20Ziqiang&rft.genre=article |