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dc.rights.licenseopenen_US
dc.contributor.authorGAILLARD, Melanie
dc.contributor.authorPARLANTI, Edith
dc.contributor.authorSOURZAC, Mahaut
hal.structure.identifierImagerie moléculaire et thérapies innovantes en oncologie [IMOTION]
dc.contributor.authorCOUILLAUD, Franck
hal.structure.identifierImagerie moléculaire et thérapies innovantes en oncologie [IMOTION]
dc.contributor.authorGENEVOIS, Coralie
dc.contributor.authorBOUTRY, Sebastien
dc.contributor.authorRIGAUD, Christian
dc.contributor.authorDAVERAT, Francoise
dc.date.accessioned2022-11-25T10:11:19Z
dc.date.available2022-11-25T10:11:19Z
dc.date.issued2022-09-26
dc.identifier.issn2045-2322en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/170392
dc.description.abstractEnAlizarin detection in fish fins is extensively employed because it is easy to use. However, in eels, the eelGFP fluorescent protein may impede the detection of the fluorescent markers in the eel tissues. The study tests the effectiveness of three of the most up-to-date alizarin-detecting technologies on the living body and fins of European glass eels (Anguilla anguilla L.). The findings demonstrated that the control group had a high autofluorescence at alizarin and eelGFP maxima bands. With fluorescence reflectance imaging (FRI), the eel living body autofluorescence impeded the detection of the marked eels. In contrast with experimental excitation-emission-matrix (EEM) fluorescence analyses, 99% of the marked eels were correctly assigned to their group from fluorescence analyses of their fin cellular contents. With epifluorometry (EPI), 100% of the marked eels were detected with the caudal fin tips when excited at 450–490 nm wavelengths due to a weaker autofluorescence signal. EEM and FRI assays unveiled an average fluorescence quenching 60% and 44% of the marked group respectively, in the alizarin and eelGFP maxima bands. The fluorescence quenching observed is discussed. Results will benefit experimental design by examining autofluorescence effects on mark detection and the development of non-invasive detection methods in this critically endangered species.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enEcology
dc.subject.enImaging
dc.subject.enMarine biology
dc.subject.enMicroscopy
dc.subject.enOptical spectroscopy
dc.title.enNew insights into detecting alizarin from autofluorescence in marked glass eels
dc.typeArticle de revueen_US
dc.identifier.doi10.1038/s41598-022-18440-0en_US
dc.subject.halSciences de l'environnementen_US
bordeaux.journalSCIENTIFIC REPORTSen_US
bordeaux.volume12en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue1en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.exportfalse
dc.rights.ccCC BYen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=SCIENTIFIC%20REPORTS&rft.date=2022-09-26&rft.volume=12&rft.issue=1&rft.eissn=2045-2322&rft.issn=2045-2322&rft.au=GAILLARD,%20Melanie&PARLANTI,%20Edith&SOURZAC,%20Mahaut&COUILLAUD,%20Franck&GENEVOIS,%20Coralie&rft.genre=article


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