PATEL, Anisha N.; ANNE, Agnès; CHOVIN, Arnaud; DEMAILLE, Christophe; GRELET, Eric; MICHON, Thierry; TAOFIFENUA, Cécilia

doi:10.1002/smll.201603163

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PATEL, Anisha N.
Laboratoire d'Electrochimie Moléculaire [LEM (UMR_7591)]

ANNE, Agnès
Laboratoire d'Electrochimie Moléculaire [LEM (UMR_7591)]

CHOVIN, Arnaud
Laboratoire d'Electrochimie Moléculaire [LEM (UMR_7591)]

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Article de revue

Ce document a été publié dans

Small. 2017, vol. 13, n° 1603163, p. pp. 1-11

Wiley-VCH Verlag

Résumé en anglais

Organizing active enzyme molecules on nanometer-sized scaffolds is a promising strategy for designing highly efficient supported catalytic systems for biosynthetic and sensing applications. This is achieved by designing model nanoscale enzymatic platforms followed by thorough analysis of the catalytic activity. Herein, the virus fd bacteriophage is considered as an enzyme nanocarrier to study the scaffolding effects on enzymatic activity. Nanoarrays of randomly oriented, or directionally patterned, fd bacteriophage virus are functionalized with the enzyme glucose oxidase (GOx), using an immunological assembly strategy, directly on a gold electrode support. Thescaffolding process on the virus capsid is monitored in situ by AFM (atomic force microscopy) imaging, while cyclic voltammetry is used to interrogate the catalytic activity of the resulting functional GOx-fd nanoarrays. Kinetic analysis reveals the ability to modulate the activity of GOx via nanocarrier patterning. The results evidence, for the first time, enhancement of the enzymatic activity due to scaffolding on a filamentous viral particle.< Réduire

Mots clés en anglais

Enzymes

Virus

DOI

http://dx.doi.org/10.1002/smll.201603163

Project ANR

Imagerie électrochimique fonctionnelle de systèmes enzymatiques multi-composants organisés sur des virus nano-gabarits - ANR-14-CE09-0009

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332