VERMA, S.; BASSIL, N.V.; VAN DE WEG, E.; HARRISON, R.J.; MONFORT, A.; HIDALGO, J.M.; AMAYA, I.; DENOYES-ROTHAN, Beatrice; MAHONEY, L.; DAVIS, T.M.; FAN, Z.; KNAPP, S.; WHITAKER, V.M.

doi:10.17660/ActaHortic.2017.1156.10

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VERMA, S.
University of Florida [Gainesville] [UF]

BASSIL, N.V.
USDA-ARS : Agricultural Research Service

VAN DE WEG, E.
Wageningen University and Research [Wageningen] [WUR]

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Communication dans un congrès

Ce document a été publié dans

Proceedings of the VIII International Strawberry Symposium, Proceedings of the VIII International Strawberry Symposium, 8. International Strawberry Symposium, 2016-08-13, Quebec. 2017, vol. 1156, p. np

International Society for Horticultural Science (ISHS)

Résumé en anglais

The Axiom® IStraw90 SNP (single nucleotide polymorphism) array was developed to enable high-throughput genotyping in allo-octoploid cultivated strawberry (Fragaria ×ananassa). However, high cost ($ 80-105 sample-1) limits throughput for certain applications. On average the IStraw90 has yielded 50 to 60% usable data points, defined as PHR (Poly High Resolution) and NMH (No Minor Homozygote) marker classes. Thus, an array is needed with a higher percentage of usable data points at a lower cost. We initiated an effort to identify IStraw90 SNP markers that were genetically mapped in one or more strawberry populations from research programs around the world. Seven programs participated in this endeavor. A total of 41,183 SNP probes were submitted to Affymetrix for quality check, 38,506 of which were accommodated on the Axiom® IStraw35 384HT design. In order to assess the performance of the Axiom® IStraw35 384HT array, 384 DNA samples from the University of Florida strawberry breeding program were assayed at a cost of $ 50 per sample, all inclusive. The performance of the array met expectations. More than 87% of markers belonged to the PHR and NMH categories. This array is expected to provide high-quality genome scanning at a more affordable price for strawberry researchers worldwide.< Réduire

Mots clés

fragaria x ananassa

plante fruitière

physiologie végétale

Mots clés en anglais

breeding

genotyping

IStraw90

single nucleotide polymorphism (SNP)

orchard crops