SALON, Christophe; AVICE, Jean-Christophe; COLOMBIE, Sophie; DIEUAIDE NOUBHANI, Martine; GALLARDO-GUERRERO, Karine; JEUDY, Christian; OURRY, Alain; PRUDENT, Marion; VOISIN, Anne-Sophie; ROLIN, Dominique

doi:10.1093/jxb/erx126

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SALON, Christophe
Agroécologie [Dijon]

AVICE, Jean-Christophe
Ecophysiologie Végétale, Agronomie et Nutritions [EVA]

COLOMBIE, Sophie
Biologie du fruit et pathologie [BFP]

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Article de revue

Ce document a été publié dans

Journal of Experimental Botany. 2017, vol. 68, n° 9, p. 2083-2098

Oxford University Press (OUP)

Résumé en anglais

Fluxes through metabolic pathways reflect the integration of genetic and metabolic regulations. While it is attractive to measure all the mRNAs (transcriptome), all the proteins (proteome), and a large number of the metabolites (metabolome) in a given cellular system, linking and integrating this information remains difficult. Measurement of metabolome-wide fluxes (termed the fluxome) provides an integrated functional output of the cell machinery and a better tool to link functional analyses to plant phenotyping. This review presents and discusses sets of methodologies that have been developed to measure the fluxome. First, the principles of metabolic flux analysis (MFA), its 'short time interval' version Inst-MFA, and of constraints-based methods, such as flux balance analysis and kinetic analysis, are briefly described. The use of these powerful methods for flux characterization at the cellular scale up to the organ (fruits, seeds) and whole-plant level is illustrated. The added value given by fluxomics methods for unravelling how the abiotic environment affects flux, the process, and key metabolic steps are also described. Challenges associated with the development of fluxomics and its integration with 'omics' for thorough plant and organ functional phenotyping are discussed. Taken together, these will ultimately provide crucial clues for identifying appropriate target plant phenotypes for breeding.< Réduire

Mots clés

physiologie végétale

plante

Mots clés en anglais

Fluxome

isotope labelling

metabolic flux analysis

metabolic models

metabolism

phenotyping.

DOI

http://dx.doi.org/10.1093/jxb/erx126

Project ANR

Centre français de phénomique végétale - ANR-11-INBS-0012

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332