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Proteolytic Post-Translational Processing of Adhesins in a Pathogenic Bacterium.

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDUBRANA-OURABAH, Marie-Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierDepartment of Molecular Genetics
dc.contributor.authorGUEGUENIAT, Julia
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierDepartment of Biology, Graduate School of Science
dc.contributor.authorBERTIN, Clothilde
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDURET NURBEL, Sybille
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBOUVERY, Nathalie
hal.structure.identifierProteome platform
dc.contributor.authorCLAVEROL, Stéphane
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLARTIGUE-PRAT, Carole
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBLANCHARD, Alain
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorRENAUDIN, Joël
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEVEN, Laure
dc.date.issued2017
dc.identifier.issn0022-2836
dc.description.abstractEnMollicutes, including mycoplasmas and spiroplasmas, have been considered as good representatives of the « minimal cell » concept: these wall-less bacteria are small in size and possess a minimal genome and restricted metabolic capacities. However, the recent discovery of the presence of post-translational modifications unknown so far, such as the targeted processing of membrane proteins of mycoplasma pathogens for human and swine, revealed a part of the hidden complexity of these microorganisms. In this study, we show that in the phytopathogen, insect-vectored Spiroplasma citri GII-3 adhesion-related protein (ScARP) adhesins are post-translationally processed through an ATP-dependent targeted cleavage. The cleavage efficiency could be enhanced in vitro when decreasing the extracellular pH or upon the addition of polyclonal antibodies directed against ScARP repeated units, suggesting that modification of the surface charge and/or ScARP conformational changes could initiate the cleavage. The two major sites for primary cleavage are localized within predicted disordered regions and do not fit any previously reported cleavage motif; in addition, the inhibition profile and the metal ion requirements indicate that this post-translational modification involves at least one non-conventional protease. Such a proteolytic process may play a role in S. citri colonization of cells of the host insect. Furthermore, our work indicates that post-translational cleavage of adhesins represents a common feature to mollicutes colonizing distinct hosts and that processing of surface antigens could represent a way to make the most out of a minimal genome.
dc.language.isoen
dc.publisherElsevier
dc.subjectpathologie végétale
dc.subjectbactérie phytopathogène
dc.subjectmollicute phytopathogène
dc.subjectsanté des plantes
dc.subject.enScARP
dc.subject.enbacterial post-translational modification
dc.subject.enmollicutes
dc.subject.ensurface antigen
dc.subject.ensurface shedding
dc.subject.enphytopathogenic bacteria
dc.subject.enplant health
dc.title.enProteolytic Post-Translational Processing of Adhesins in a Pathogenic Bacterium.
dc.typeArticle de revue
dc.identifier.doi10.1016/j.jmb.2017.05.004
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalJournal of Molecular Biology
bordeaux.page1889-1902
bordeaux.volume429
bordeaux.issue12
bordeaux.peerReviewedoui
hal.identifierhal-01605883
hal.version1
hal.popularnon
hal.audienceNon spécifiée
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01605883v1
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