Proteolytic Post-Translational Processing of Adhesins in a Pathogenic Bacterium.
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | DUBRANA-OURABAH, Marie-Pierre | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
hal.structure.identifier | Department of Molecular Genetics | |
dc.contributor.author | GUEGUENIAT, Julia | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
hal.structure.identifier | Department of Biology, Graduate School of Science | |
dc.contributor.author | BERTIN, Clothilde | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | DURET NURBEL, Sybille | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | BOUVERY, Nathalie | |
hal.structure.identifier | Proteome platform | |
dc.contributor.author | CLAVEROL, Stéphane | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | LARTIGUE-PRAT, Carole | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | BLANCHARD, Alain | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | RENAUDIN, Joël | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | BEVEN, Laure | |
dc.date.issued | 2017 | |
dc.identifier.issn | 0022-2836 | |
dc.description.abstractEn | Mollicutes, including mycoplasmas and spiroplasmas, have been considered as good representatives of the « minimal cell » concept: these wall-less bacteria are small in size and possess a minimal genome and restricted metabolic capacities. However, the recent discovery of the presence of post-translational modifications unknown so far, such as the targeted processing of membrane proteins of mycoplasma pathogens for human and swine, revealed a part of the hidden complexity of these microorganisms. In this study, we show that in the phytopathogen, insect-vectored Spiroplasma citri GII-3 adhesion-related protein (ScARP) adhesins are post-translationally processed through an ATP-dependent targeted cleavage. The cleavage efficiency could be enhanced in vitro when decreasing the extracellular pH or upon the addition of polyclonal antibodies directed against ScARP repeated units, suggesting that modification of the surface charge and/or ScARP conformational changes could initiate the cleavage. The two major sites for primary cleavage are localized within predicted disordered regions and do not fit any previously reported cleavage motif; in addition, the inhibition profile and the metal ion requirements indicate that this post-translational modification involves at least one non-conventional protease. Such a proteolytic process may play a role in S. citri colonization of cells of the host insect. Furthermore, our work indicates that post-translational cleavage of adhesins represents a common feature to mollicutes colonizing distinct hosts and that processing of surface antigens could represent a way to make the most out of a minimal genome. | |
dc.language.iso | en | |
dc.publisher | Elsevier | |
dc.subject | pathologie végétale | |
dc.subject | bactérie phytopathogène | |
dc.subject | mollicute phytopathogène | |
dc.subject | santé des plantes | |
dc.subject.en | ScARP | |
dc.subject.en | bacterial post-translational modification | |
dc.subject.en | mollicutes | |
dc.subject.en | surface antigen | |
dc.subject.en | surface shedding | |
dc.subject.en | phytopathogenic bacteria | |
dc.subject.en | plant health | |
dc.title.en | Proteolytic Post-Translational Processing of Adhesins in a Pathogenic Bacterium. | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1016/j.jmb.2017.05.004 | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale | |
bordeaux.journal | Journal of Molecular Biology | |
bordeaux.page | 1889-1902 | |
bordeaux.volume | 429 | |
bordeaux.issue | 12 | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-01605883 | |
hal.version | 1 | |
hal.popular | non | |
hal.audience | Non spécifiée | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-01605883v1 | |
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