Proteolytic Post-Translational Processing of Adhesins in a Pathogenic Bacterium.
BERTIN, Clothilde
Biologie du fruit et pathologie [BFP]
Department of Biology, Graduate School of Science
See more >
Biologie du fruit et pathologie [BFP]
Department of Biology, Graduate School of Science
BERTIN, Clothilde
Biologie du fruit et pathologie [BFP]
Department of Biology, Graduate School of Science
< Reduce
Biologie du fruit et pathologie [BFP]
Department of Biology, Graduate School of Science
Language
en
Article de revue
This item was published in
Journal of Molecular Biology. 2017, vol. 429, n° 12, p. 1889-1902
Elsevier
English Abstract
Mollicutes, including mycoplasmas and spiroplasmas, have been considered as good representatives of the « minimal cell » concept: these wall-less bacteria are small in size and possess a minimal genome and restricted ...Read more >
Mollicutes, including mycoplasmas and spiroplasmas, have been considered as good representatives of the « minimal cell » concept: these wall-less bacteria are small in size and possess a minimal genome and restricted metabolic capacities. However, the recent discovery of the presence of post-translational modifications unknown so far, such as the targeted processing of membrane proteins of mycoplasma pathogens for human and swine, revealed a part of the hidden complexity of these microorganisms. In this study, we show that in the phytopathogen, insect-vectored Spiroplasma citri GII-3 adhesion-related protein (ScARP) adhesins are post-translationally processed through an ATP-dependent targeted cleavage. The cleavage efficiency could be enhanced in vitro when decreasing the extracellular pH or upon the addition of polyclonal antibodies directed against ScARP repeated units, suggesting that modification of the surface charge and/or ScARP conformational changes could initiate the cleavage. The two major sites for primary cleavage are localized within predicted disordered regions and do not fit any previously reported cleavage motif; in addition, the inhibition profile and the metal ion requirements indicate that this post-translational modification involves at least one non-conventional protease. Such a proteolytic process may play a role in S. citri colonization of cells of the host insect. Furthermore, our work indicates that post-translational cleavage of adhesins represents a common feature to mollicutes colonizing distinct hosts and that processing of surface antigens could represent a way to make the most out of a minimal genome.Read less <
Keywords
pathologie végétale
bactérie phytopathogène
mollicute phytopathogène
santé des plantes
English Keywords
ScARP
bacterial post-translational modification
mollicutes
surface antigen
surface shedding
phytopathogenic bacteria
plant health
Origin
Hal importedCollections