RAVELONANDRO, Michel; SCORZA, Ralph; BRIARD, Pascal

doi:10.3390/plants8120565

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

RAVELONANDRO, Michel
Biologie du fruit et pathologie [BFP]

SCORZA, Ralph
USDA-ARS : Agricultural Research Service

BRIARD, Pascal
Biologie du fruit et pathologie [BFP]

Langue

Article de revue

Ce document a été publié dans

Plants. 2019, vol. 8, n° 12, p. 565

MDPI

Résumé en anglais

We developed an innovative RNAi concept based on two gene constructs built from the capsid gene (CP) cistron of the Plum pox virus (PPV) genome. First, designated as amiCPRNA, a potential molecule interfering with PPV genome translation and the second one is the ami-siCPRNA to target viral genome translation and PPV RNA replication. Following the previous engineering of these constructs in an experimental herbaceous host, they were introduced into Prunus domestica (plum tree) genome. Previously propagated onto a susceptible rootstock, these clones were graft-inoculated with PPV. After four dormancy cycles, and consistent with our experience of PPV infection, some clones showed a common phenomenon of silencing that can differ between the detailed plant phenotypes. Three different phenotypes were developed by the amisiCPRNA clones. First, the high resistance character shown by the amisiCPRNA plum-7 that was similar to the resistance expressed by HoneySweet plum. Secondly, a recovery reaction was developed by the two other amisiCPRNA plum-3 and plum-4 that differed from the rest, characterized as susceptible clones, among these were the amiCPRNA plums. Having assessed the behavior of these plums versus the herbaceous host accumulating the similar form of RNAi: ami-, si-, and ami-siRNA, challenging assays in perennials consistently reflect the natural context of viral genome targeting< Réduire

Mots clés

Prunus domestica

RNAi

Mots clés en anglais

plum pox virus stability

recovery

resistance

silencing