LEMAIRE-CHAMLEY, Martine; MOUNET, Fabien; DEBORDE, Catherine; MAUCOURT, Mickael; JACOB, Daniel; MOING, Annick

doi:10.3390/metabo9050093

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

LEMAIRE-CHAMLEY, Martine
Biologie du fruit et pathologie [BFP]

MOUNET, Fabien
Biologie du fruit et pathologie [BFP]

DEBORDE, Catherine
Biologie du fruit et pathologie [BFP]
Plateforme Bordeaux Metabolome

Langue

Article de revue

Ce document a été publié dans

Metabolites. 2019-05-09, vol. 9, n° 5, p. 93

MDPI

Résumé en anglais

Fruit is a complex organ containing seeds and several interconnected tissues with dedicated roles. However, most biochemical or molecular studies about fleshy fruit development concern the entire fruit, the fruit without seeds, or pericarp only. We studied tomato (Solanum lycopersicum) fruit at four stages of development (12, 20, 35, and 45 days post-anthesis). We separated the seeds and the other tissues, exocarp, mesocarp, columella with placenta and locular tissue, and analyzed them individually using proton NMR metabolomic profiling for the quantification of major polar metabolites, enzymatic analysis of starch, and LC-DAD analysis of isoprenoids. Pericarp tissue represented about half of the entire fruit mass only. The composition of each fruit tissue changed during fruit development. An ANOVA-PCA highlighted common, and specific metabolite trends between tissues e.g., higher contents of chlorogenate in locular tissue and of starch in columella. Euclidian distances based on compositional data showed proximities within and between tissues. Several metabolic regulations differed between tissues as revealed by the comparison of metabolite networks based on correlations between compounds. This work stressed the role of specific tissues less studied than pericarp but that impact fruit organoleptic quality including its shape and taste, and fruit processing quality.< Réduire

Mots clés en anglais

NMR metabolomic profiling

fruit development

isoprenoids

seeds

tomato

fruit tissues

DOI

http://dx.doi.org/10.3390/metabo9050093

Project ANR

Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332