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Genome-scale model of Rhodotorula toruloides metabolism.

hal.structure.identifierChalmers University of Technology [Gothenburg, Sweden]
hal.structure.identifierDepartment of Molecular Sciences
dc.contributor.authorTIUKOVA, Ievgeniia A
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPRIGENT, Sylvain
hal.structure.identifierChalmers University of Technology [Gothenburg, Sweden]
dc.contributor.authorNIELSEN, Jens
hal.structure.identifierDepartment of Molecular Sciences
dc.contributor.authorSANDGREN, Mats
hal.structure.identifierChalmers University of Technology [Gothenburg, Sweden]
dc.contributor.authorKERKHOVEN, Eduard J
dc.date.issued2019
dc.identifier.issn0006-3592
dc.description.abstractEnThe basidiomycete red yeast Rhodotorula toruloides is a promising platform organism for production of biooils. We present rhto-GEM, the first genome-scale model (GEM) of R. toruloides metabolism, that was largely reconstructed using RAVEN toolbox. The model includes 852 genes, 2,731 reactions, and 2,277 metabolites, while lipid metabolism is described using the SLIMEr formalism allowing direct integration of lipid class and acyl chain experimental distribution data. The simulation results confirmed that the R. toruloides model provides valid growth predictions on glucose, xylose, and glycerol, while prediction of genetic engineering targets to increase production of linolenic acid, triacylglycerols, and carotenoids identified genes-some of which have previously been engineered to successfully increase production. This renders rtho-GEM valuable for future studies to improve the production of other oleochemicals of industrial relevance including value-added fatty acids and carotenoids, in addition to facilitate system-wide omics-data analysis in R. toruloides. Expanding the portfolio of GEMs for lipid-accumulating fungi contributes to both understanding of metabolic mechanisms of the oleaginous phenotype but also uncover particularities of the lipid production machinery in R. toruloides.
dc.language.isoen
dc.publisherWiley
dc.subjectRhodotorula toruloides
dc.subject.engenome-scale model
dc.subject.enmetabolism
dc.subject.enyeast
dc.title.enGenome-scale model of Rhodotorula toruloides metabolism.
dc.typeArticle de revue
dc.identifier.doi10.1002/bit.27162
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalBiotechnology and Bioengineering
bordeaux.page3396-3408
bordeaux.volume116
bordeaux.issue12
bordeaux.peerReviewedoui
hal.identifierhal-02624789
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02624789v1
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