Genome-scale model of Rhodotorula toruloides metabolism.
TIUKOVA, Ievgeniia A
Chalmers University of Technology [Gothenburg, Sweden]
Department of Molecular Sciences
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Chalmers University of Technology [Gothenburg, Sweden]
Department of Molecular Sciences
TIUKOVA, Ievgeniia A
Chalmers University of Technology [Gothenburg, Sweden]
Department of Molecular Sciences
< Leer menos
Chalmers University of Technology [Gothenburg, Sweden]
Department of Molecular Sciences
Idioma
en
Article de revue
Este ítem está publicado en
Biotechnology and Bioengineering. 2019, vol. 116, n° 12, p. 3396-3408
Wiley
Resumen en inglés
The basidiomycete red yeast Rhodotorula toruloides is a promising platform organism for production of biooils. We present rhto-GEM, the first genome-scale model (GEM) of R. toruloides metabolism, that was largely reconstructed ...Leer más >
The basidiomycete red yeast Rhodotorula toruloides is a promising platform organism for production of biooils. We present rhto-GEM, the first genome-scale model (GEM) of R. toruloides metabolism, that was largely reconstructed using RAVEN toolbox. The model includes 852 genes, 2,731 reactions, and 2,277 metabolites, while lipid metabolism is described using the SLIMEr formalism allowing direct integration of lipid class and acyl chain experimental distribution data. The simulation results confirmed that the R. toruloides model provides valid growth predictions on glucose, xylose, and glycerol, while prediction of genetic engineering targets to increase production of linolenic acid, triacylglycerols, and carotenoids identified genes-some of which have previously been engineered to successfully increase production. This renders rtho-GEM valuable for future studies to improve the production of other oleochemicals of industrial relevance including value-added fatty acids and carotenoids, in addition to facilitate system-wide omics-data analysis in R. toruloides. Expanding the portfolio of GEMs for lipid-accumulating fungi contributes to both understanding of metabolic mechanisms of the oleaginous phenotype but also uncover particularities of the lipid production machinery in R. toruloides.< Leer menos
Palabras clave
Rhodotorula toruloides
Palabras clave en inglés
genome-scale model
metabolism
yeast
Orígen
Importado de HalCentros de investigación