KOLONIUK, Igor; SARKISOVA, Tatiana; PETRZIK, Karel; LENZ, Ondřej; PŘIBYLOVÁ, Jaroslava; FRÁNOVÁ, Jana; LOTOS, Leonidas; BETA, Christina; KATSIANI, Asimina; CANDRESSE, Thierry; MALIOGKA, Varvara I

doi:10.3390/v10040204

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KOLONIUK, Igor
Biology Centre of the Czech Academy of Sciences [BIOLOGY CENTRE CAS]

SARKISOVA, Tatiana
Biology Centre of the Czech Academy of Sciences [BIOLOGY CENTRE CAS]

PETRZIK, Karel
Biology Centre of the Czech Academy of Sciences [BIOLOGY CENTRE CAS]

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Viruses. 2018, vol. 10, n° 4, p. 204

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Résumé en anglais

During their lifetime, perennial woody plants are expected to face multiple infection events. Furthermore, multiple genotypes of individual virus species may co-infect the same host. This may eventually lead to a situation where plants harbor complex communities of viral species/strains. Using high-throughput sequencing, we describe co-infection of sweet and sour cherry trees with diverse genomic variants of two closely related viruses, namely prunus virus F (PrVF) and cherry virus F (CVF). Both viruses are most homologous to members of the Fabavirus genus (Secoviridae family). The comparison of CVF and PrVF RNA2 genomic sequences suggests that the two viruses may significantly differ in their expression strategy. Indeed, similar to comoviruses, the smaller genomic segment of PrVF, RNA2, may be translated in two collinear proteins while CVF likely expresses only the shorter of these two proteins. Linked with the observation that identity levels between the coat proteins of these two viruses are significantly below the family species demarcation cut-off, these findings support the idea that CVF and PrVF represent two separate Fabavirus species.< Réduire

Mots clés

phylogenetics

plant virus

prunus

Mots clés en anglais

fabavirus

high-throughput sequencing

intrahost variability

novel species