BEUVE, Monique; HILY, Jean-Michel; ALLIAUME, Antoine; REINBOLD, Catherine; LE MAGUET, Jean; CANDRESSE, Thierry; HERRBACH, Etienne; LEMAIRE, Olivier

doi:10.1007/s00705-018-3949-9

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BEUVE, Monique
Santé de la vigne et qualité du vin [SVQV]

HILY, Jean-Michel
Santé de la vigne et qualité du vin [SVQV]

ALLIAUME, Antoine
Santé de la vigne et qualité du vin [SVQV]

Langue

Article de revue

Ce document a été publié dans

Archives of Virology. 2018, vol. 163, p. 2937-2946

Springer Verlag

Résumé en anglais

We have characterized the virome of a grapevine Pinot Noir accession (P70) that displayed, over the year, very stable and strong leafroll symptoms. For this, we have used two extraction methods (dsRNA and total RNA) coupled with the high throughput sequencing (HTS) Illumina technique. While a great disparity in viral sequences were observed, both approaches gave similar results, revealing a very complex infection status. Five virus and viroid isolates [Grapevine leafroll-associated viruse-1 (GLRaV-1), Grapevine virus A (GVA), Grapevine rupestris stem pitting-associated virus (GRSPaV), Hop stunt viroid (HSVd) and Grapevine yellow speckle viroid 1 (GYSVd1)] were detected in P70 with a grand total of eleven variants being identified and de novo assembled. A comparison between both extraction methods regarding their power to detect viruses and the ease of genome assembly is also provided.< Réduire

Mots clés en anglais

virome

Grapevine rupestris stem pitting-associated virus

Hop stunt viroid

Grapevine yellow speckle viroid 1

DOI

http://dx.doi.org/10.1007/s00705-018-3949-9

Project ANR

Nanobodies: le couteau Suisse de la virologie de la vigne - ANR-14-CE19-0018

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332