The plant WEE1 kinase is involved in checkpoint control activation in nematode induced galls.
hal.structure.identifier | Institut Sophia Agrobiotech [ISA] | |
dc.contributor.author | CABRAL, Danila | |
hal.structure.identifier | Institut Sophia Agrobiotech [ISA] | |
hal.structure.identifier | جامعة عين شمس - Ain Shams University [Cairo] [ASU] | |
dc.contributor.author | BANORA, Mohamed Youssef | |
hal.structure.identifier | Institut Sophia Agrobiotech [ISA] | |
hal.structure.identifier | Laboratório de Interação Molecular Planta-Praga | |
dc.contributor.author | DIJAIR ANTONINO, José | |
hal.structure.identifier | Institut Sophia Agrobiotech [ISA] | |
hal.structure.identifier | Embrapa Recursos Genéticos e Biotecnologia = Embrapa Genetic Resources & Biotechnology [CENARGEN] | |
dc.contributor.author | RODIUC, Natalia | |
hal.structure.identifier | Institut Sophia Agrobiotech [ISA] | |
dc.contributor.author | VIEIRA, Paulo | |
hal.structure.identifier | Institut Sophia Agrobiotech [ISA] | |
hal.structure.identifier | Embrapa Recursos Genéticos e Biotecnologia = Embrapa Genetic Resources & Biotechnology [CENARGEN] | |
dc.contributor.author | R. COELHO, Roberta | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | CHEVALIER, Christian | |
hal.structure.identifier | Department of Plant Biotechnology and Genetics | |
hal.structure.identifier | Flanders Institute for Biotechnology | |
dc.contributor.author | EEKHOUT, Thomas | |
hal.structure.identifier | Institut Sophia Agrobiotech [ISA] | |
dc.contributor.author | ENGLER, Gilbert | |
hal.structure.identifier | Department of Plant Biotechnology and Genetics | |
hal.structure.identifier | Flanders Institute for Biotechnology | |
dc.contributor.author | DE VEYLDER, Lieven | |
hal.structure.identifier | Embrapa Recursos Genéticos e Biotecnologia = Embrapa Genetic Resources & Biotechnology [CENARGEN] | |
dc.contributor.author | GROSSI-DE-SA, Maria Fatima | |
hal.structure.identifier | Institut Sophia Agrobiotech [ISA] | |
dc.contributor.author | DE ALMEIDA ENGLER, Janice | |
dc.date.issued | 2020 | |
dc.identifier.issn | 0028-646X | |
dc.description.abstractEn | Galls induced by plant-parasitic nematodes involve a hyperactivation of the plant mitotic and endocycle machinery for their profit. Dedifferentiation of host root cells includes drastic cellular and molecular readjustments. In such background, potential DNA damage in the genome of gall cells is eminent. We questioned if DNA damage checkpoints activation followed by DNA repair occurred, or was eventually circumvented, in nematode-induced galls. Galls display transcriptional activation of the DNA damage checkpoint kinase WEE1, correlated with its protein localization in the nuclei. The promoter of the stress marker gene SMR7 was evaluated under the WEE1-knockout background. Drugs inducing DNA damage and a marker for DNA repair, PARP1 were used to understand mechanisms that might cope with DNA damage in galls. Our functional study revealed that gall cells lacking WEE1 conceivably entered mitosis prematurely disturbing the cell cycle despite the loss of genome integrity. The disrupted nuclei phenotype in giant cells hinted to the accumulation of mitotic defects. As well, WEE1-knockout in Arabidopsis and downregulation in tomato repressed infection and reproduction of root-knot nematodes. Together with data on DNA damaging drugs, we suggest a conserved function for WEE1 controlling a G1/S cell cycle arrest in response to replication defect in galls. | |
dc.language.iso | en | |
dc.publisher | Wiley | |
dc.subject | cell cycle | |
dc.subject | galls | |
dc.subject.en | Arabidopsis thaliana | |
dc.subject.en | Solanum lycopersicum | |
dc.subject.en | WEE1 kinase | |
dc.subject.en | checkpoint control | |
dc.subject.en | root-knot nematode | |
dc.title.en | The plant WEE1 kinase is involved in checkpoint control activation in nematode induced galls. | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1111/nph.16185 | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale | |
bordeaux.journal | New Phytologist | |
bordeaux.page | 430-447 | |
bordeaux.volume | 225 | |
bordeaux.issue | 1 | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-02629362 | |
hal.version | 1 | |
hal.popular | non | |
hal.audience | Internationale | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-02629362v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=New%20Phytologist&rft.date=2020&rft.volume=225&rft.issue=1&rft.spage=430-447&rft.epage=430-447&rft.eissn=0028-646X&rft.issn=0028-646X&rft.au=CABRAL,%20Danila&BANORA,%20Mohamed%20Youssef&DIJAIR%20ANTONINO,%20Jos%C3%A9&RODIUC,%20Natalia&VIEIRA,%20Paulo&rft.genre=article |
Files in this item
Files | Size | Format | View |
---|---|---|---|
There are no files associated with this item. |