CABRAL, Danila; BANORA, Mohamed Youssef; DIJAIR ANTONINO, José; RODIUC, Natalia; VIEIRA, Paulo; R. COELHO, Roberta; CHEVALIER, Christian; EEKHOUT, Thomas; ENGLER, Gilbert; DE VEYLDER, Lieven; GROSSI-DE-SA, Maria Fatima; DE ALMEIDA ENGLER, Janice

doi:10.1111/nph.16185

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CABRAL, Danila
Institut Sophia Agrobiotech [ISA]

BANORA, Mohamed Youssef
Institut Sophia Agrobiotech [ISA]
Ain Shams University [ASU]

DIJAIR ANTONINO, José
Institut Sophia Agrobiotech [ISA]
Laboratório de Interação Molecular Planta-Praga

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New Phytologist. 2020, vol. 225, n° 1, p. 430-447

Wiley

Résumé en anglais

Galls induced by plant-parasitic nematodes involve a hyperactivation of the plant mitotic and endocycle machinery for their profit. Dedifferentiation of host root cells includes drastic cellular and molecular readjustments. In such background, potential DNA damage in the genome of gall cells is eminent. We questioned if DNA damage checkpoints activation followed by DNA repair occurred, or was eventually circumvented, in nematode-induced galls. Galls display transcriptional activation of the DNA damage checkpoint kinase WEE1, correlated with its protein localization in the nuclei. The promoter of the stress marker gene SMR7 was evaluated under the WEE1-knockout background. Drugs inducing DNA damage and a marker for DNA repair, PARP1 were used to understand mechanisms that might cope with DNA damage in galls. Our functional study revealed that gall cells lacking WEE1 conceivably entered mitosis prematurely disturbing the cell cycle despite the loss of genome integrity. The disrupted nuclei phenotype in giant cells hinted to the accumulation of mitotic defects. As well, WEE1-knockout in Arabidopsis and downregulation in tomato repressed infection and reproduction of root-knot nematodes. Together with data on DNA damaging drugs, we suggest a conserved function for WEE1 controlling a G1/S cell cycle arrest in response to replication defect in galls.< Réduire

Mots clés

cell cycle

galls

Mots clés en anglais

Arabidopsis thaliana

Solanum lycopersicum

WEE1 kinase

checkpoint control

root-knot nematode