SCHIECK, Elise; LARTIGUE, Carole; FREY, Joachim; VOZZA, Nicolas; HEGERMANN, Jan; MILLER, Rachel A; VALGUARNERA, Ezequiel; MURIUKI, Cecilia; MEENS, Jochen; NENE, Vish; NAESSENS, Jan; WEBER, Johann; LOWARY, Todd L; VASHEE, Sanjay; FELDMAN, Mario F; JORES, Joerg

doi:10.1111/mmi.13213

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SCHIECK, Elise
International Livestock Research Institute [CGIAR, Nairobi] [ILRI]

LARTIGUE, Carole
Biologie du fruit et pathologie [BFP]

FREY, Joachim
Universität Bern = University of Bern = Université de Berne [UNIBE]

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Ce document a été publié dans

Molecular Microbiology. 2016, vol. 99, n° 1, p. 55-70

Wiley

Résumé en anglais

Mycoplasma mycoides subsp. capri (Mmc) and subsp. mycoides (Mmm) are important ruminant pathogens worldwide causing diseases such as pleuropneumonia, mastitis and septicaemia. They express galactofuranose residues on their surface, but their role in pathogenesis has not yet been determined. The M. mycoides genomes contain up to several copies of the glf gene, which encodes an enzyme catalyzing the last step in the synthesis of galactofuranose. We generated a deletion of the glf gene in a strain of Mmc using genome transplantation and tandem repeat endonuclease coupled cleavage (TREC) with yeast as an intermediary host for the genome editing. As expected, the resulting YCp1.1-Δglf strain did not produce the galactofuranose containing glycans as shown by immunoblots and immuno-electronmicroscopy employing a galactofuranose specific monoclonal antibody. The mutant lacking galactofuranose exhibited a decreased growth rate and a significantly enhanced adhesion to small ruminant cells. The mutant was also "leaking" as revealed by a β-galactosidase-based assay employing a membrane impermeable substrate. These findings indicate that galactofuranose-containing polysaccharides conceal adhesins and are important for membrane integrity. Unexpectedly, the mutant strain showed increased serum resistance.< Réduire

Mots clés

galactan

galactofuranose

membrane

synthetic genomics

Mots clés en anglais

Mycoplasma mycoides

UDP-galactopyranose mutase

adhesion

carbohydrate