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Effector polymorphisms of the sunflower downy mildew pathogen <em>Plasmopara Halstedii</em> and their use to identify pathotypes from field isolates

hal.structure.identifierLaboratoire des interactions plantes micro-organismes [LIPM]
dc.contributor.authorGASCUEL, Quentin
hal.structure.identifierLaboratoire des interactions plantes micro-organismes [LIPM]
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBORDAT, Amandine
hal.structure.identifierLaboratoire des interactions plantes micro-organismes [LIPM]
dc.contributor.authorSALLET, Erika
hal.structure.identifierLaboratoire des interactions plantes micro-organismes [LIPM]
dc.contributor.authorPOUILLY, Nicolas
hal.structure.identifierLaboratoire des interactions plantes micro-organismes [LIPM]
dc.contributor.authorCARRERE, Sebastien
hal.structure.identifierLaboratoire des interactions plantes micro-organismes [LIPM]
dc.contributor.authorROUX, Fabrice
hal.structure.identifierLaboratoire des interactions plantes micro-organismes [LIPM]
dc.contributor.authorVINCOURT, Patrick
hal.structure.identifierLaboratoire des interactions plantes micro-organismes [LIPM]
dc.contributor.authorGODIARD, Laurence
dc.date.issued2016
dc.identifier.issn1932-6203
dc.description.abstractEnThe obligate biotroph oomycete Plasmopara halstedii causes downy mildew on sunflower crop, Helianthus annuus. The breakdown of several Pl resistance genes used in sunflower hybrids over the last 25 years came along with the appearance of new Pl. halstedii isolates showing modified virulence profiles. In oomycetes, two classes of effector proteins, key players of pathogen virulence, are translocated into the host: RXLR and CRN effectors. We identified 54 putative CRN or RXLR effector genes from transcriptomic data and analyzed their genetic diversity in seven Pl. halstedii pathotypes representative of the species variability. Pl. halstedii effector genes were on average more polymorphic at both the nucleic and protein levels than random non-effector genes, suggesting a potential adaptive dynamics of pathogen virulence over the last 25 years. Twenty-two KASP (Competitive Allele Specific PCR) markers designed on polymorphic effector genes were genotyped on 35 isolates belonging to 14 Pl. halstedii pathotypes. Polymorphism analysis based on eight KASP markers aims at proposing a determination key suitable to classify the eight multi-isolate pathotypes into six groups. This is the first report of a molecular marker set able to discriminate Pl. halstedii pathotypes based on the polymorphism of pathogenicity effectors. Compared to phenotypic tests handling living spores used until now to discriminate Pl. halstedii pathotypes, this set of molecular markers constitutes a first step in faster pathotype diagnosis of Pl. halstedii isolates. Hence, emerging sunflower downy mildew isolates could be more rapidly characterized and thus, assessment of plant resistance breakdown under field conditions should be improved.
dc.description.sponsorshipUniversité Fédérale de Toulouse - ANR-11-IDEX-0002
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectRxlr effectors
dc.subjectEvolution
dc.subject.enSingle nucleotide polymorphisms
dc.subject.enHelianthus-annuus L
dc.subject.enPhytophthora-infestans
dc.subject.enDisease resistance
dc.subject.enMultiple introductions
dc.subject.enOomycete effectors
dc.subject.enSequence
dc.subject.enVirulence
dc.title.enEffector polymorphisms of the sunflower downy mildew pathogen <em>Plasmopara Halstedii</em> and their use to identify pathotypes from field isolates
dc.typeArticle de revue
dc.identifier.doi10.1371/journal.pone.0148513
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPLoS ONE
bordeaux.page1-19
bordeaux.volume11
bordeaux.issue2
bordeaux.peerReviewedoui
hal.identifierhal-02632845
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02632845v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=PLoS%20ONE&amp;rft.date=2016&amp;rft.volume=11&amp;rft.issue=2&amp;rft.spage=1-19&amp;rft.epage=1-19&amp;rft.eissn=1932-6203&amp;rft.issn=1932-6203&amp;rft.au=GASCUEL,%20Quentin&amp;BORDAT,%20Amandine&amp;SALLET,%20Erika&amp;POUILLY,%20Nicolas&amp;CARRERE,%20Sebastien&amp;rft.genre=article


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