WATTELET-BOYER, Valérie; BROCARD, Lysiane; JONSSON, Kristoffer; ESNAY, Nicolas; JOUBES, Jérôme; DOMERGUE, Frédéric; MONGRAND, Sébastien; RAIKHEL, Natasha; BHALERAO, Rishikesh P.; MOREAU, Patrick; BOUTTÉ, Yohann

doi:10.1038/ncomms12788

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WATTELET-BOYER, Valérie
Laboratoire de biogenèse membranaire [LBM]
Université de Bordeaux [UB]
Institut National de la Recherche Agronomique [INRA]

BROCARD, Lysiane
Bordeaux Imaging Center [BIC]
Centre National de la Recherche Scientifique [CNRS]
Institut National de la Santé et de la Recherche Médicale [INSERM]

JONSSON, Kristoffer
Umea Plant Science Center [UPSC]

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Article de revue

Ce document a été publié dans

Nature Communications. 2016, vol. 7, p. 1-15

Nature Publishing Group

Résumé en anglais

The post-Golgi compartment trans-Golgi Network (TGN) is a central hub divided into multiple subdomains hosting distinct trafficking pathways, including polar delivery to apical membrane. Lipids such as sphingolipids and sterols have been implicated in polar trafficking from the TGN but the underlying mechanisms linking lipid composition to functional polar sorting at TGN subdomains remain unknown. Here we demonstrate that sphingolipids with alpha-hydroxylated acyl-chains of at least 24 carbon atoms are enriched in secretory vesicle subdomains of the TGN and are critical for de novo polar secretory sorting of the auxin carrier PIN2 to apical membrane of Arabidopsis root epithelial cells. We show that sphingolipid acyl-chain length influences the morphology and interconnections of TGN-associated secretory vesicles. Our results uncover that the sphingolipids acyl-chain length links lipid composition of TGN subdomains with polar secretory trafficking of PIN2 to apical membrane of polarized epithelial cells.< Réduire

DOI

http://dx.doi.org/10.1038/ncomms12788

Project ANR

Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010

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Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332