RECHT, Lee; TÖPFER, Nadine; BATUSHANSKY, Albert; SIKRON, Noga; ZARKA, Aliza; GIBON, Yves; NIKOLOSKI, Zoran; FAIT, Aaron; BOUSSIBA, Sammy

doi:10.1074/jbc.M114.555144

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RECHT, Lee
Ben-Gurion University of the Negev [BGU]

TÖPFER, Nadine
Max Planck Institute of Molecular Plant Physiology [MPI-MP]

BATUSHANSKY, Albert
Ben-Gurion University of the Negev [BGU]

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Article de revue

Ce document a été publié dans

Journal of Biological Chemistry. 2014, vol. 289, n° 44, p. 30387-30403

American Society for Biochemistry and Molecular Biology

Résumé en anglais

The green alga Haematococcus pluvialis accumulates large amounts of the antioxidant astaxanthin under inductive stress conditions, such as nitrogen starvation. The response to nitrogen starvation and high-light leads to the accumulation of carbohydrates and fatty acids, as well as increased activity of the tricarboxylic acid cycle. Although the behavior of individual pathways is well-investigated, little is known about the systemic effects of the stress-response mechanism. Here we present time-resolved metabolite, enzyme activity, and physiological data that capture the metabolic response of H. pluvialis under nitrogen starvation and high-light. The data were integrated into a putative genome-scale model of the green alga to in silico test the hypothesis of underlying carbon partitioning. The model-based hypothesis testing reinforces the involvement of starch degradation to support fatty acid synthesis in the later stages of the stress response. In addition, our findings support a possible mechanism for the involvement of the increased activity of the tricarboxylic acid cycle in carbon repartitioning. Finally, the in vitro experiments and the in silico modeling presented here emphasize the predictive power of large-scale integrative approaches to pinpoint metabolic adjustment to changing environments.< Réduire

Mots clés

Haematococcus pluvialis

metabolomics

Mots clés en anglais

algae

carbohydrate metabolism

computational biology

constraint-based modeling

data integration

fatty acid metabolism

nitrogen starvation

systems biology