RODRIGUEZ-MEDINA, Caren; BOISSINOT, Sylvaine; CHAPUIS, Sophie; GEREIGE, Dalya; RASTEGAR, Maryam; ERDINGER, Monique; REVERS, Frederic; ZIEGLER-GRAFF, Véronique; BRAULT, Véronique

doi:10.1016/j.virol.2015.08.031

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RODRIGUEZ-MEDINA, Caren
Santé de la vigne et qualité du vin [SVQV]

BOISSINOT, Sylvaine
Santé de la vigne et qualité du vin [SVQV]

CHAPUIS, Sophie
Institut de Biologie Moléculaire des Plantes [IBMP]

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Ce document a été publié dans

Virology. 2015, vol. 486, p. 44-53

Elsevier

Résumé en anglais

Turnip yellows virus (TuYV), a phloem-limited virus, encodes a 74 kDa protein known as the readthrough protein (RT) involved in virus movement. We show here that a TuYV mutant deleted of the C-terminal part of the RT protein (TuYV-ΔRTCter) was affected in long-distance trafficking in a host-specific manner. By using the C-terminal domain of the RT protein as a bait in a yeast two-hybrid screen of a phloem cDNA library from Arabidopsis thaliana we identified the calcineurin B-like protein-interacting protein kinase-7 (AtCIPK7). Transient expression of a GFP:CIPK7 fusion protein in virus-inoculated Nicotiana benthamiana leaves led to local increase of wild-type TuYV accumulation, but not that of TuYV-ΔRTCter. Surprisingly, elevated virus titer in inoculated leaves did not result in higher TuYV accumulation in systemic leaves, which indicates that virus long-distance movement was not affected. Since GFP:CIPK7 was localized in or near plasmodesmata, CIPK7 could negatively regulate TuYV export from infected cells.< Réduire

Mots clés

Plant;

Mots clés en anglais

Polerovirus;

Phloem;

Movement;

Kinase;

CIPK