LARTIGUE, Carole; LEBAUDY, Anne; BLANCHARD, Alain; YACOUBI, Basma El; ROSE, Simon; GROSJEAN, Henri; DOUTHWAITE, Stephen

doi:10.1093/nar/gku518

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

http://creativecommons.org/licenses/by-nc/

LARTIGUE, Carole
Biologie du fruit et pathologie [BFP]

LEBAUDY, Anne
Biologie du fruit et pathologie [BFP]

BLANCHARD, Alain
Biologie du fruit et pathologie [BFP]

Langue

Article de revue

Ce document a été publié dans

Nucleic Acids Research. 2014, vol. 42, n° 12, p. 8073-8082

Oxford University Press

Résumé en anglais

Efficient protein synthesis in all organisms requires the post-transcriptional methylation of specific ribosomal ribonucleic acid (rRNA) and transfer RNA (tRNA) nucleotides. The methylation reactions are almost invariably catalyzed by enzymes that use S-adenosylmethionine (AdoMet) as the methyl group donor. One noteworthy exception is seen in some bacteria, where the conserved tRNA methylation at m(5)U54 is added by the enzyme TrmFO using flavin adenine dinucleotide together with N(5),N(10)-methylenetetrahydrofolate as the one-carbon donor. The minimalist bacterium Mycoplasma capricolum possesses two homologs of trmFO, but surprisingly lacks the m(5)U54 tRNA modification. We created single and dual deletions of the trmFO homologs using a novel synthetic biology approach. Subsequent analysis of the M. capricolum RNAs by mass spectrometry shows that the TrmFO homolog encoded by Mcap0476 specifically modifies m(5)U1939 in 23S rRNA, a conserved methylation catalyzed by AdoMet-dependent enzymes in all other characterized bacteria. The Mcap0476 methyltransferase (renamed RlmFO) represents the first folate-dependent flavoprotein seen to modify ribosomal RNA.< Réduire

Métadonnées

Licence d’utilisation du document

The flavoprotein Mcap0476 (RlmFO) catalyzes m5U1939 modification in <em>Mycoplasma capricolum</em> 23S rRNA

Langue

Ce document a été publié dans

Résumé en anglais

DOI

Origine

Unités de recherche