JUST, Daniel; GARCIA, Virginie; FERNANDEZ, Lucie; BRES, Cécile; MAUXION, Jean-Philippe; PETIT, Johann; JORLY, Joana; ASSALI, Julien; BOURNONVILLE, Celine; FERRAND, Carine; BALDET, Pierre; LEMAIRE-CHAMLEY, Martine; MORI, Kentaro; OKABE, Yoshihiro; ARIZUMI, Tohru; EZURA, Hiroshi; ROTHAN, Christophe

doi:10.5511/plantbiotechnology.13.0622a

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JUST, Daniel
Biologie du fruit et pathologie [BFP]

GARCIA, Virginie
Biologie du fruit et pathologie [BFP]

FERNANDEZ, Lucie
Biologie du fruit et pathologie [BFP]

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Article de revue

Ce document a été publié dans

Plant Biotechnology. 2013, vol. 30, n° 3, p. 225-231

Japanese Society for Plant Cell and Molecular Biology

Résumé en anglais

Tomato is currently the model plant for fleshy fruit development and for Solanaceae species. Recent genomic approaches including transcriptome, proteome and metabolome analyses and genetic mapping have produced a wealth of candidate genes whose function needs to be assessed. The recent development in model and crop plants of TILLING (Targeting Induced Local Lesions IN Genomes), which reveals allelic series corresponding to several independent point mutations, and the current availability of deep sequencing tools further increase the interest of generating artificially-induced genetic diversity in tomato. We describe here the generation and use of EMS (ethyl methanesulfonate) tomato mutants in the miniature cultivar Micro-Tom and provide as example the identification of new fruit size and morphology mutants. We further propose new deep sequencing-based strategies for the discovery of mutations underlying phenotypic variations observed in mutant collections that will considerably increase the interest of exploiting Micro-Tom mutant collections for gene discovery in tomato.< Réduire

Mots clés

mutant

point mutation

model system

Mots clés en anglais

deep sequencing

phenotype

database

tomato

tilling

forward genetic

fruit development

cultivated tomato

provide insight

induced mutation

cuticle formation

reverse genetic

candidate gene

arabidopsis