ILLA, Eudald; EDUARDO, Iban; AUDERGON, Jean Marc; BARALE, Francesca; DIRLEWANGER, Elisabeth; XIONGWEI LI, -; MOING, Annick; LAMBERT, Patrick; LE DANTEC, Loick; ZHONGSHAN GAO, -; POESSEL, Jean-Luc; POZZI, Carlo; ROSSINI, Laura; VECCHIETTI, Alberto; ARUS, Pere; HOWAD, Werner

doi:10.1007/s11032-010-9518-x

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ILLA, Eudald
Institut de Recerca i Tecnologia Agroalimentàries = Institute of Agrifood Research and Technology [IRTA]

EDUARDO, Iban
Institut de Recerca i Tecnologia Agroalimentàries = Institute of Agrifood Research and Technology [IRTA]
Parco Tecnologico Padano

AUDERGON, Jean Marc
Génétique et Amélioration des Fruits et Légumes [GAFL]

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Ce document a été publié dans

Molecular Breeding. 2011, vol. 28, n° 4, p. 667-682

Springer Verlag

Résumé en anglais

To identify genes involved in the expression of a trait using the candidate gene (CG) approach, the genome positions of the maximum number of genes which potentially cause the observed phenotypic variability needs to be known. This position is compared with that of major genes or quantitative trait loci (QTLs) for this character, with co-location of the CG and major gene or QTL indicating possible cause and effect situations. In the present study we selected 273 sequences from EST collections, corresponding to CGs from metabolic pathways affecting fruit growth and maturity, texture, sugar and organic acid content, aroma and color, and mapped them in the Prunus reference map (TxE) based on an interspecific almond x peach F2 population. We used the bin-mapping approach, where only eight plants: six of the TxE progeny plus one of the parents and the F1 hybrid, are used to determine the position of a marker. This strategy was very efficient, with 206 CGs mapped, based mainly on the segregation of one or more single-nucleotide polymorphisms. These CGs were located throughout the Prunus genome and are a resource for genetic analysis in stone fruit (peach, plum, apricot and cherry) and almond. Co-locations between CGs and major genes or QTLs responsible for natural variability of fruit quality characters in Prunus were identified using the available information on their positions.< Réduire

Mots clés

est collection

cartographie genetique

plant sciences

Mots clés en anglais

candidate gene

bin mapping

snp variability

prunus reference map

genetics and heredity

agronomy

horticulture