BALDACCI, Fabien; CHANG, Christine; MAUCOURT, Mickael; DEBORDE, Catherine; HOPKINS, Julie; LECOMTE, Philippe; BERNILLON, Stéphane; BROUQUISSE, Renaud; MOING, Annick; ABAD, Pierre; HÉROUART, Didier; PUPPO, Alain; FAVERY, Bruno; FRENDO, Pierre

doi:10.1371/journal.ppat.1002471

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

BALDACCI, Fabien
Interactions Biotiques et Santé Végétale [IBSV]

CHANG, Christine
Interactions Biotiques et Santé Végétale [IBSV]

MAUCOURT, Mickael
Biologie du fruit et pathologie [BFP]

Langue

Article de revue

Ce document a été publié dans

PLoS Pathogens. 2012, vol. 8, n° 1, p. e1002471

Public Library of Science

Résumé en anglais

Root-knot nematodes (RKN) are obligatory plant parasitic worms that establish and maintain an intimate relationship with their host plants. During a compatible interaction, RKN induce the redifferentiation of root cells into multinucleate and hypertrophied giant cells essential for nematode growth and reproduction. These metabolically active feeding cells constitute the exclusive source of nutrients for the nematode. Detailed analysis of glutathione (GSH) and homoglutathione (hGSH) metabolism demonstrated the importance of these compounds for the success of nematode infection in Medicago truncatula. We reported quantification of GSH and hGSH and gene expression analysis showing that (h)GSH metabolism in neoformed gall organs differs from that in uninfected roots. Depletion of (h)GSH content impaired nematode egg mass formation and modified the sex ratio. In addition, gene expression and metabolomic analyses showed a substantial modification of starch and γ-aminobutyrate metabolism and of malate and glucose content in (h)GSH-depleted galls. Interestingly, these modifications did not occur in (h)GSH-depleted roots. These various results suggest that (h)GSH have a key role in the regulation of giant cell metabolism. The discovery of these specific plant regulatory elements could lead to the development of new pest management strategies against nematodes< Réduire

Mots clés

giant cell

RELATION PLANTE-MICROORGANISME

Mots clés en anglais

nodule

gene expression

Medicago trunculata