ZAGRAI, I.; RAVELONANDRO, Michel; GABOREANU, I.; FERENCZ, B.; SCORZA, R.; ZAGRAI, L.; KELEMEN, B.; PAMFIL, D.; POPESCU, O.

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

ZAGRAI, I.
Fruit Research and Development Station Bistrita

RAVELONANDRO, Michel
Biologie du fruit et pathologie [BFP]

GABOREANU, I.
University of Agricultural Sciences and Veterinary Medicine

Langue

Article de revue

Ce document a été publié dans

Journal of Plant Pathology. 2011, vol. 93, n° 1, p. 159-165

Springer

Résumé en anglais

The serological and genetic variability of Plum pox virus (PPV) isolates from transgenic plum trees expressing the PPV coat protein gene and conventional plums was analyzed. PPV isolates were characterized serologically by TAS-ELISA using PPV-D and PPV-M specific monoclonal antibodies and by molecular typing across three genomic regions as well as RFLP analysis of the 3' terminus of the CP gene. PCR products spanning the (Cter)CP and (Cter)NIb-(Nter)CP regions were sequenced. Sequence information revealed no significant difference between serological and molecular features of PPV isolates from transgenic and conventional plums that were growing together in experimental orchards for 6-8 years. These results show that transgenic plums do not promote the emergence of new PPV variants under field conditions.< Réduire

Mots clés

GENETIQUE

Mots clés en anglais

PATHOGEN DERIVED RESISTANCE

GMO

CP GENE

SAFETY ISSUE

RECOMBINANT VIRUSES

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332