VILAINE, Francoise; KERCHEV, Pavel Ivanov; CLÉMENT, Gilles; BATAILLER, Brigitte; CAYLA, Thibaud; BILL, Laurence; GISSOT, Lionel; DINANT, Sylvie

doi:10.1105/tpc.113.111849

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VILAINE, Francoise
Institut Jean-Pierre Bourgin [IJPB]

KERCHEV, Pavel Ivanov
Institut Jean-Pierre Bourgin [IJPB]

CLÉMENT, Gilles
Institut Jean-Pierre Bourgin [IJPB]

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The Plant cell. 2013, vol. 25, n° 5, p. 1689 -1708

American Society of Plant Biologists (ASPB)

Résumé en anglais

The complex process of phloem sugar transport involves symplasmic and apoplasmic events. We characterized Arabidopsis thaliana lines ectopically expressing a phloem-specific gene encoding NDR1/HIN1-like26 (NHL26), a putative membrane protein. NHL26 overexpressor plants grew more slowly than wild-type plants, accumulated high levels of carbohydrates in mature leaves, and had a higher shoot biomass, contrasting with slower root growth and a lower seed yield. Similar effects were observed when NHL26 was overexpressed in companion cells, under the control of a companion cell-specific promoter. The soluble sugar content of the phloem sap and sink organs was lower than that in the wild type, providing evidence of a sugar export defect. This was confirmed in a phloem-export assay with the symplastic tracer carboxyfluorescein diacetate. Leaf sugar accumulation was accompanied by higher organic acid, amino acid, and protein contents, whereas analysis of the metabolite profile of phloem sap exudate revealed no change in amino acid or organic acid content, indicating a specific effect on sugar export. NHL26 was found to be located in the phloem plasmodesmata and the endoplasmic reticulum. These findings reveal that NHL26 accumulation affects either the permeability of plasmodesmata or sugar signaling in companion cells, with a specific effect on sugar export.< Réduire

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virus movement protein

embryogenesis abundant proteins

promote carbohydrate export

tissue specific expression

transgenic potato plants

maize leaves

gene expression

amino acids

seed yield

sucrose transporters

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Increased expression of a phloem membrane protein encoded by NHL26 alters phloem export and sugar partitioning in [i]Arabidopsis[/i]

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