TAVERT-ROUDET, Genevieve; ABDUL RAZZAK, Anas; DOUBLET, Bénédicte; WALTER, Jocelyne; DELAUNAY, Thierry; MICHON, Thierry; LE GALL, Olivier; CANDRESSE, Thierry

doi:10.1099/vir.0.035881-0

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TAVERT-ROUDET, Genevieve
Biologie du fruit et pathologie [BFP]

ABDUL RAZZAK, Anas
Biologie du fruit et pathologie [BFP]

DOUBLET, Bénédicte
Biologie du fruit et pathologie [BFP]

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Journal of General Virology. 2012, vol. 93, n° 1, p. 184-193

Microbiology Society

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Recessive resistance to lettuce mosaic virus (LMV) is conferred in lettuce by the mo1 gene, encoding the eukaryotic translation initiation factor 4E (eIF4E). The C terminus of the viral cylindrical inclusion helicase (CI-Cter), together with the VPg, is involved directly in overcoming mo1 resistance. In this study, recombinant LMV VPg and CI-Cter proteins from wild-type or resistance-breaking isolates were expressed and purified from Escherichia coli. The allelic forms of eIF4E from susceptible or resistant lettuce cultivars were produced similarly and these proteins were used in ELISA-based assays to demonstrate the in vitro binding of the various forms of LMV CI-Cter to both lettuce eIF4E and LMV VPg proteins. All combinations tested displayed significant and specific interactions, and the interaction between the C-terminal part of the LMV CI and eIF4E was confirmed in vivo in bimolecular fluorescence complementation assays. Higher interaction signals for both CI–eIF4E and CI–VPg were observed for LMV-E, indicating that the eIF4E interaction network involving CI and VPg appears to be stronger in the case of this resistance-breaking isolate. This could suggest the need for a minimal interaction threshold for infection success in resistant lettuce, but more precise measurement of the interaction parameters linking eIF4E, VPg and CI is needed in order to reinforce such a hypothesis.< Réduire

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VIROLOGIE

BIOLOGIE MOLECULAIRE

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The C terminus of lettuce mosaic potyvirus cylindrical inclusion helicase interacts with the viral VPg and with lettuce translation eukaryotic initiation factor 4E

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