BOULILA-ZOGHLAMI, Latifa; GALLUSCI, Philippe; HOLZER, Frances M.; BASSET, Gilles J.; DJEBALI, Whabi; CHAIBI, Wided; WALLING, Linda L.; BROUQUISSE, Renaud

doi:10.1007/s00425-011-1468-y

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BOULILA-ZOGHLAMI, Latifa
Université de Tunis

GALLUSCI, Philippe
Biologie du fruit et pathologie [BFP]

HOLZER, Frances M.
University of California [Riverside] [UC Riverside]

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Article de revue

Ce document a été publié dans

Planta. 2011, vol. 234, n° 4, p. 857-863

Springer Verlag

Résumé en anglais

The effects of cadmium (Cd) on aminopeptidase (AP) activities and Leucine-AP (LAP) expression were investigated in the roots of tomato (Solanum lycopersicum L., var Ibiza) plants. Three-week-old plants were grown for 10 days in the presence of 0.3-300 μM Cd and compared to control plants grown in the absence of Cd. AP activities were measured using six different p-nitroanilide (p-NA) substrates. Leu, Met, Arg, Pro and Lys hydrolyzing activities increased in roots of Cd-treated plants, while Phe-pNA cleavage was not enhanced after Cd treatments. The use of peptidase inhibitors showed that most of the Leu-pNA hydrolyzing activity was related to one or several metallo-APs. Changes in Lap transcripts, protein and activities were measured in the roots of 0 and 30-μM Cd-treated plants. LapA transcript levels increased in Cd-treated roots, whereas LapN RNAs levels were not modified. To assess amount of Leu-pNA hydrolyzing activity associated with the hexameric LAPs, LAP activity was measured following immunoprecipitation with a LAP polyclonal antiserum. LAP activity increased in Cd-treated roots. There was a corresponding increase in LAP-A protein levels detected in 2D-immunoblots. The role of LAP-A in the proteolytic response to Cd stress is discussed.< Réduire

Mots clés

BIOLOGIE VEGETALE

Mots clés en anglais

LAP-A