RUMBOU, Artemis; CANDRESSE, Thierry; MARAIS, Armelle; SVANELLA-DUMAS, Laurence; LANDGRAF, Maria; VON BARGEN, Susanne; BÜTTNER, Carmen

doi:10.1371/journal.pone.0221834

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RUMBOU, Artemis
Humboldt-Universität zu Berlin = Humboldt University of Berlin = Université Humboldt de Berlin [HU Berlin]

CANDRESSE, Thierry
Biologie du fruit et pathologie [BFP]

MARAIS, Armelle
Biologie du fruit et pathologie [BFP]

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Article de revue

Ce document a été publié dans

PLoS ONE. 2020, vol. 15, n° 6, p. e0221834

Public Library of Science

Résumé en anglais

To unravel the virome in birch trees of German and Finnish origin exhibiting symptoms of birch leaf-roll disease (BRLD), high-throughput sequencing (HTS) was employed. In total five viruses, among which three were so far unknown, were detected by RNAseq. One to five virus variants were identified in the transcriptome of individual trees. The novel viruses were genetically-fully or partially-characterized, belonging to the genera Carlavirus, Idaeovirus and Capillovirus and are tentatively named birch carlavirus, birch idaeovirus, and birch capillovirus, respectively. The recently discovered birch leafroll-associated virus was systematically detected by HTS in symptomatic seedlings but not in symptomless ones. The new carlavirus was detected only in one of the three symptomatic seedlings. The novel puta-tive Capillovirus was detected in all seedlings-irrespective of their BLRD status-while the Idaeovirus was identified in a plant without leaf symptoms at the time of sampling. Further efforts are needed to complete Koch's postulates and to clarify the possible association of the detected viruses with the BLR disease. Our study elucidates the viral population in single birch seedlings and provides a comprehensive overview for the diversities of the viral communities they harbor, to date.< Réduire

Mots clés

arbre forestier

Bouleau

Séquençage à haut débit

Virome

détection

virus

Carlavirus

Idaeovirus

Capillovirus

virus phytopathogène

Virologie végétale

Santé des plantes

Mots clés en anglais

leaf roll virus