Molecular Viral Diagnosis and Sanitation of Yam Genetic Resources: Implications for Safe Yam Germplasm Exchange
hal.structure.identifier | Agrosystèmes tropicaux [ASTRO] | |
dc.contributor.author | UMBER, Marie | |
hal.structure.identifier | Biologie et Génétique des Interactions Plante-Parasite [UMR BGPI] | |
hal.structure.identifier | Département Systèmes Biologiques [Cirad-BIOS] | |
dc.contributor.author | FILLOUX, Denis | |
hal.structure.identifier | Agrosystèmes tropicaux [ASTRO] | |
dc.contributor.author | GÉLABALE, Suzia | |
hal.structure.identifier | Biologie et Génétique des Interactions Plante-Parasite [UMR BGPI] | |
dc.contributor.author | GOMEZ, Rose-Marie | |
hal.structure.identifier | Biologie du fruit et pathologie [BFP] | |
dc.contributor.author | MARAIS, Armelle | |
hal.structure.identifier | Biologie et Génétique des Interactions Plante-Parasite [UMR BGPI] | |
dc.contributor.author | GALLET, Séverine | |
hal.structure.identifier | Biologie et Génétique des Interactions Plante-Parasite [UMR BGPI] | |
dc.contributor.author | GAMIETTE, Franciane | |
hal.structure.identifier | Biologie et Génétique des Interactions Plante-Parasite [UMR BGPI] | |
dc.contributor.author | PAVIS, Claudie | |
hal.structure.identifier | Amélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP] | |
hal.structure.identifier | Département Systèmes Biologiques [Cirad-BIOS] | |
dc.contributor.author | TEYCHENEY, Pierre-Yves | |
dc.date.issued | 2020 | |
dc.identifier.issn | 1999-4915 | |
dc.description.abstractEn | Yam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report on the implementation of reliable and cost-effective PCR-based detection tools targeting eight different yam-infecting viruses. Viral indexing of the in vitro yam collection maintained by the Biological Resources Center for Tropical Plants (BRC-TP) in Guadeloupe (French West Indies) unveiled a high prevalence of potyviruses, badnaviruses, Dioscorea mosaic associated virus (DMaV) and yam asymptomatic virus 1 (YaV1) and a high level of coinfections. Infected yam accessions were subjected to a combination of thermotherapy and meristem culture. Sanitation levels were monitored using PCR-based and high-throughput sequencing-based diagnosis, confirming the efficacy and reliability of PCR-based detection tools. Sanitation rates were highly variable depending on viruses. Sixteen accessions were successfully sanitized, paving the way to safe yam germplasm exchanges and the implementation of clean seed production programs worldwide. | |
dc.language.iso | en | |
dc.publisher | MDPI | |
dc.rights.uri | http://creativecommons.org/licenses/by/ | |
dc.subject | virologie végétale | |
dc.subject | virus phytopathogène | |
dc.subject | santé des plantes | |
dc.subject | pathologie végétale | |
dc.subject.en | molecular diagnosis | |
dc.subject.en | yam | |
dc.subject.en | viruses | |
dc.subject.en | sanitation | |
dc.subject.en | germplasm conservation and dissemination | |
dc.title.en | Molecular Viral Diagnosis and Sanitation of Yam Genetic Resources: Implications for Safe Yam Germplasm Exchange | |
dc.type | Article de revue | |
dc.identifier.doi | 10.3390/v12101101 | |
dc.subject.hal | Sciences du Vivant [q-bio] | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie | |
bordeaux.journal | Viruses | |
bordeaux.page | 1101 | |
bordeaux.volume | 12 | |
bordeaux.issue | 10 | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-02955296 | |
hal.version | 1 | |
hal.popular | non | |
hal.audience | Internationale | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-02955296v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Viruses&rft.date=2020&rft.volume=12&rft.issue=10&rft.spage=1101&rft.epage=1101&rft.eissn=1999-4915&rft.issn=1999-4915&rft.au=UMBER,%20Marie&FILLOUX,%20Denis&G%C3%89LABALE,%20Suzia&GOMEZ,%20Rose-Marie&MARAIS,%20Armelle&rft.genre=article |
Fichier(s) constituant ce document
Fichiers | Taille | Format | Vue |
---|---|---|---|
Il n'y a pas de fichiers associés à ce document. |