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Molecular Viral Diagnosis and Sanitation of Yam Genetic Resources: Implications for Safe Yam Germplasm Exchange

hal.structure.identifierAgrosystèmes tropicaux [ASTRO]
dc.contributor.authorUMBER, Marie
hal.structure.identifierBiologie et Génétique des Interactions Plante-Parasite [UMR BGPI]
hal.structure.identifierDépartement Systèmes Biologiques [Cirad-BIOS]
dc.contributor.authorFILLOUX, Denis
hal.structure.identifierAgrosystèmes tropicaux [ASTRO]
dc.contributor.authorGÉLABALE, Suzia
hal.structure.identifierBiologie et Génétique des Interactions Plante-Parasite [UMR BGPI]
dc.contributor.authorGOMEZ, Rose-Marie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMARAIS, Armelle
hal.structure.identifierBiologie et Génétique des Interactions Plante-Parasite [UMR BGPI]
dc.contributor.authorGALLET, Séverine
hal.structure.identifierBiologie et Génétique des Interactions Plante-Parasite [UMR BGPI]
dc.contributor.authorGAMIETTE, Franciane
hal.structure.identifierBiologie et Génétique des Interactions Plante-Parasite [UMR BGPI]
dc.contributor.authorPAVIS, Claudie
hal.structure.identifierAmélioration génétique et adaptation des plantes méditerranéennes et tropicales [UMR AGAP]
hal.structure.identifierDépartement Systèmes Biologiques [Cirad-BIOS]
dc.contributor.authorTEYCHENEY, Pierre-Yves
dc.date.issued2020
dc.identifier.issn1999-4915
dc.description.abstractEnYam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report on the implementation of reliable and cost-effective PCR-based detection tools targeting eight different yam-infecting viruses. Viral indexing of the in vitro yam collection maintained by the Biological Resources Center for Tropical Plants (BRC-TP) in Guadeloupe (French West Indies) unveiled a high prevalence of potyviruses, badnaviruses, Dioscorea mosaic associated virus (DMaV) and yam asymptomatic virus 1 (YaV1) and a high level of coinfections. Infected yam accessions were subjected to a combination of thermotherapy and meristem culture. Sanitation levels were monitored using PCR-based and high-throughput sequencing-based diagnosis, confirming the efficacy and reliability of PCR-based detection tools. Sanitation rates were highly variable depending on viruses. Sixteen accessions were successfully sanitized, paving the way to safe yam germplasm exchanges and the implementation of clean seed production programs worldwide.
dc.language.isoen
dc.publisherMDPI
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectvirologie végétale
dc.subjectvirus phytopathogène
dc.subjectsanté des plantes
dc.subjectpathologie végétale
dc.subject.enmolecular diagnosis
dc.subject.enyam
dc.subject.enviruses
dc.subject.ensanitation
dc.subject.engermplasm conservation and dissemination
dc.title.enMolecular Viral Diagnosis and Sanitation of Yam Genetic Resources: Implications for Safe Yam Germplasm Exchange
dc.typeArticle de revue
dc.identifier.doi10.3390/v12101101
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale/Phytopathologie et phytopharmacie
bordeaux.journalViruses
bordeaux.page1101
bordeaux.volume12
bordeaux.issue10
bordeaux.peerReviewedoui
hal.identifierhal-02955296
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02955296v1
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