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MRSI vs CEST MRI to understand tomato metabolism in ripening fruit: is there a better contrast?

hal.structure.identifierQualité des Produits Animaux [QuaPA]
dc.contributor.authorPAGÈS, Guilhem
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorDEBORDE, Catherine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorLEMAIRE-CHAMLEY, Martine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorMOING, Annick
hal.structure.identifierQualité des Produits Animaux [QuaPA]
dc.contributor.authorBONNY, J.-M.
dc.date.issued2021
dc.identifier.issn1618-2642
dc.description.abstractEnBesides structural information, magnetic resonance imaging (MRI) is crucial to reveal the presence and gradients of metabolites in organs constituted of several tissues. In plant science, such knowledge is key to better understand fruit development and metabolism. Routine methods based on fixation for cytological studies or dissection for metabolite measurements induce biases and plant sample destruction. Magnetic resonance spectroscopy imaging (MSRI) leads to one NMR spectrum per pixel while chemical exchange saturation transfer (CEST) MRI allows mapping metabolites having exchangeable protons. As both methods present different advantages and drawbacks, we compared them to map metabolites in ripe tomato fruits. We demonstrated that MRSI was difficult to interpret due to large spatial chemical shift variations while CEST MRI produced promising image mapping of the main carbohydrates and amino acids. It showed that glucose/fructose was mostly located in the locular tissue, whereas glutamate/glutamine/GABA was found inside the columella.Graphical abstract.
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
dc.language.isoen
dc.publisherSpringer Verlag
dc.subjectMétabolisme
dc.subjectPlante fruitière
dc.subjectdéveloppement du fruit
dc.subject.enChemical exchange saturation transfer (CEST)
dc.subject.enMagnetic resonance spectroscopy imaging (MRSI)
dc.subject.enMetabolites
dc.subject.enRipe fruit
dc.subject.enSugars
dc.subject.enTomato.
dc.title.enMRSI vs CEST MRI to understand tomato metabolism in ripening fruit: is there a better contrast?
dc.typeArticle de revue
dc.identifier.doi10.1007/s00216-020-03101-w
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalAnalytical and Bioanalytical Chemistry
bordeaux.page1251-1257
bordeaux.volume413
bordeaux.peerReviewedoui
hal.identifierhal-03101885
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03101885v1
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