MRSI vs CEST MRI to understand tomato metabolism in ripening fruit: is there a better contrast?
Idioma
en
Article de revue
Este ítem está publicado en
Analytical and Bioanalytical Chemistry. 2021, vol. 413, p. 1251-1257
Springer Verlag
Resumen en inglés
Besides structural information, magnetic resonance imaging (MRI) is crucial to reveal the presence and gradients of metabolites in organs constituted of several tissues. In plant science, such knowledge is key to better ...Leer más >
Besides structural information, magnetic resonance imaging (MRI) is crucial to reveal the presence and gradients of metabolites in organs constituted of several tissues. In plant science, such knowledge is key to better understand fruit development and metabolism. Routine methods based on fixation for cytological studies or dissection for metabolite measurements induce biases and plant sample destruction. Magnetic resonance spectroscopy imaging (MSRI) leads to one NMR spectrum per pixel while chemical exchange saturation transfer (CEST) MRI allows mapping metabolites having exchangeable protons. As both methods present different advantages and drawbacks, we compared them to map metabolites in ripe tomato fruits. We demonstrated that MRSI was difficult to interpret due to large spatial chemical shift variations while CEST MRI produced promising image mapping of the main carbohydrates and amino acids. It showed that glucose/fructose was mostly located in the locular tissue, whereas glutamate/glutamine/GABA was found inside the columella.Graphical abstract.< Leer menos
Palabras clave
Métabolisme
Plante fruitière
développement du fruit
Palabras clave en inglés
Chemical exchange saturation transfer (CEST)
Magnetic resonance spectroscopy imaging (MRSI)
Metabolites
Ripe fruit
Sugars
Tomato.
Proyecto ANR
Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
Orígen
Importado de HalCentros de investigación