DE MARCO, Federica; BATAILLER, Brigitte; THORPE, Michael R.; RAZAN, Frédérique; LE HIR, Rozenn; VILAINE, Françoise; BOUCHEREAU, Alain; MARTIN-MAGNIETTE, Marie-Laure; EVEILLARD, Sandrine; DINANT, Sylvie

doi:10.3390/ijms22020745

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DE MARCO, Federica
Institut Jean-Pierre Bourgin [IJPB]

BATAILLER, Brigitte
Biologie du fruit et pathologie [BFP]

THORPE, Michael R.
Plant Sciences Division, Research School of Biology, The Australian National University

Langue

Article de revue

Ce document a été publié dans

International Journal of Molecular Sciences. 2021-01-13, vol. 22, n° 2, p. 745

MDPI

Résumé en anglais

Phytoplasmas inhabit phloem sieve elements and cause abnormal growth and altered sugar partitioning. However, how they interact with phloem functions is not clearly known. The phloem responses were investigated in tomatoes infected by " Phytoplasma solani" at the beginning of the symptomatic stage, the first symptoms appearing in the newly emerged leaf at the stem apex. Antisense lines impaired in the phloem sucrose transporters SUT1 and SUT2 were included. In symptomatic sink leaves, leaf curling was associated with higher starch accumulation and the expression of defense genes. The analysis of leaf midribs of symptomatic leaves indicated that transcript levels for genes acting in the glycolysis and peroxisome metabolism differed from these in noninfected plants. The phytoplasma also multiplied in the three lower source leaves, even if it was not associated with the symptoms. In these leaves, the rate of phloem sucrose exudation was lower for infected plants. Metabolite profiling of phloem sap-enriched exudates revealed that glycolate and aspartate levels were affected by the infection. Their levels were also affected in the noninfected - and -antisense lines. The findings suggest the role of sugar transporters in the responses to infection and describe the consequences of impaired sugar transport on the primary metabolism.< Réduire

Mots clés

Mollicute

Bactérie

Pathologie végétale

Santé des Plantes

Mots clés en anglais

phloem

peroxisome

sugar metabolism

glyoxylate

glycolate

source-sink relationships

carbon allocation

photorespiration

metabolome

plant-pathogen interaction

phytoplasma

defense

DOI

http://dx.doi.org/10.3390/ijms22020745

Project ANR

Saclay Plant Sciences - ANR-10-LABX-0040
Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay - ANR-17-EURE-0007
Développement d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332