ARRICAU-BOUVERY, Nathalie; DURET, Sybille; DUBRANA, Marie-Pierre; DESQUÉ, Delphine; EVEILLARD, Sandrine; BROCARD, Lysiane; MALEMBIC-MAHER, Sylvie; FOISSAC, Xavier

doi:10.1038/s41598-021-90809-z

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ARRICAU-BOUVERY, Nathalie
Biologie du fruit et pathologie [BFP]

DURET, Sybille
Biologie du fruit et pathologie [BFP]

DUBRANA, Marie-Pierre
Biologie du fruit et pathologie [BFP]

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Article de revue

Ce document a été publié dans

Scientific Reports. 2021-05-27, vol. 11, n° 1, p. 11222

Nature Publishing Group

Résumé en anglais

The flavescence dorée phytoplasma undergoes a propagative cycle in its insect vectors by first interacting with the insect cell surfaces, primarily in the midgut lumen and subsequently in the salivary glands. Adhesion of flavescence dorée phytoplasma to insect cells is mediated by the adhesin VmpA. We hypothesize that VmpA may have lectin-like activity, similar to several adhesins of bacteria that invade the insect gut. We first demonstrated that the luminal surface of the midgut and the basal surface of the salivary gland cells of the natural vector Scaphoideus titanus and those of the experimental vector Euscelidius variegatus were differentially glycosylated. Using ELISA, inhibition and competitive adhesion assays, and protein overlay assays in the Euva-6 insect cell line, we showed that the protein VmpA binds insect proteins in a lectin-like manner. In conclusion, the results of this study indicate that N-acetylglucosamine and mannose present on the surfaces of the midgut and salivary glands serve as recognition sites for the phytoplasma adhesin VmpA.< Réduire

Mots clés

flavescence dorée

Mollicute phytopathogène

phytoplasme

bactérie phytopathogène

insecte vecteur

interaction

pathologie végétale

santé des plantes

Scaphoideus titanus

Euscelidius variegatus

Mots clés en anglais

adhesin Vmpa