ANCÍN, María; LARRAYA, Luis; FLOREZ-SARASA, Igor; BÉNARD, Camille; FERNÁNDEZ-SAN MILLÁN, Alicia; VERAMENDI, Jon; GIBON, Yves; FERNIE, Alisdair; ARANJUELO, Iker; FARRAN, Inmaculada

doi:10.1093/jxb/erab193

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ANCÍN, María
Universidad Pública de Navarra [Espagne] = Public University of Navarra [UPNA]

LARRAYA, Luis
Universidad Pública de Navarra [Espagne] = Public University of Navarra [UPNA]

FLOREZ-SARASA, Igor
Max-Planck-Institut für Molekulare Pflanzenphysiologie [MPI-MP]

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Article de revue

Ce document a été publié dans

Journal of Experimental Botany. 2021, vol. 72, n° 13, p. 4949-4964

Oxford University Press (OUP)

Résumé en anglais

In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism and its coordination is fundamental for plant growth and development. In the present work, the influence of thioredoxin (Trx) m on C and N partitioning was studied using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants, indicating an up-regulation of the GS-GOGAT pathway. Trx m-overexpressing plants specifically displayed increased activity and stability of glutamine synthetase in tobacco plants. Moreover, higher photorespiration and nitrate accumulation were determined in these plants relative to the untransformed control, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, the combined results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts.< Réduire

Mots clés

Metabolisme

Plante

Mots clés en anglais

GS-GOGAT pathway

Thioredoxin

carbon metabolism

chloroplast

glutamine synthetase

nitrogen metabolism

photorespiration

DOI

http://dx.doi.org/10.1093/jxb/erab193

Project ANR

Centre français de phénomique végétale - ANR-11-INBS-0012

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332