MASSART, Sebastien; OLMOS, Antonio; JIJAKLI, Haissam; CANDRESSE, Thierry

doi:10.1016/j.virusres.2014.03.029

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MASSART, Sebastien
Agro-Bio Tech

OLMOS, Antonio
Instituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research [IVIA]

JIJAKLI, Haissam
Agro-Bio Tech

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Virus Research. 2014, vol. 188, p. 90-96

Elsevier

Résumé en anglais

The ability to provide a fast, inexpensive and reliable diagnostic for any given viral infection is a key parameter in efforts to fight and control these ubiquitous pathogens. The recent developments of high-throughput sequencing (also called Next Generation Sequencing - NGS) technologies and bioinformatics have drastically changed the research on viral pathogens. It is now raising a growing interest for virus diagnostics. This review provides a snapshot vision on the current use and impact of high throughput sequencing approaches in plant virus characterization. More specifically, this review highlights the potential of these new technologies and their interplay with current protocols in the future of molecular diagnostic of plant viruses. The current limitations that will need to be addressed for a wider adoption of high-throughput sequencing in plant virus diagnostics are thoroughly discussed.< Réduire

Mots clés en anglais

virus detection

virus diagnostic

high throughput sequencing

next generation sequencing

trends

plant virus

plant certification

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Current impact and future directions of high throughput sequencing in plant virus diagnostics

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