DESTAILLEUR, Alice; POUCET, Théo; CABASSON, Cecile; ALONSO, Ana Paula; COCURON, Jean-Christophe; LARBAT, Romain; VERCAMBRE, G.; COLOMBIE, Sophie; PÉTRIACQ, Pierre; ANDRIEU, Marie Hélène; BEAUVOIT, Bertrand; GIBON, Yves; DIEUAIDE-NOUBHANI, Martine

doi:10.3390/metabo11120848

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DESTAILLEUR, Alice
Biologie du fruit et pathologie [BFP]

POUCET, Théo
Biologie du fruit et pathologie [BFP]

CABASSON, Cecile
Biologie du fruit et pathologie [BFP]
Plateforme Bordeaux Metabolome

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Article de revue

Ce document a été publié dans

Metabolites. 2021-12, vol. 11, n° 12, p. 848

MDPI

Résumé en anglais

During its development, the leaf undergoes profound metabolic changes to ensure, among other things, its growth. The subcellular metabolome of tomato leaves was studied at four stages of leaf development, with a particular emphasis on the composition of the vacuole, a major actor of cell growth. For this, leaves were collected at different positions of the plant, corresponding to different developmental stages. Coupling cytology approaches to non-aqueous cell fractionation allowed to estimate the subcellular concentrations of major compounds in the leaves. The results showed major changes in the composition of the vacuole across leaf development. Thus, sucrose underwent a strong allocation, being mostly located in the vacuole at the beginning of development and in the cytosol at maturity. Furthermore, these analyses revealed that the vacuole, rather rich in secondary metabolites and sugars in the growth phases, accumulated organic acids thereafter. This result suggests that the maintenance of the osmolarity of the vacuole of mature leaves would largely involve inorganic molecules.< Réduire

Mots clés en anglais

vacuole

non-aqueous fractionation

subcellular metabolome

tomato leaf

DOI

http://dx.doi.org/10.3390/metabo11120848

Project ANR

Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
Centre français de phénomique végétale

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332