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hal.structure.identifierUniversiteit Gent = Ghent University [UGENT]
hal.structure.identifierCenter for Plant Systems Biology [PSB Center]
dc.contributor.authorSWINNEN, Gwen
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMAUXION, Jean-Philippe
hal.structure.identifierUniversiteit Gent = Ghent University [UGENT]
hal.structure.identifierCenter for Plant Systems Biology [PSB Center]
dc.contributor.authorBAEKELANDT, Alexandra
hal.structure.identifierUniversiteit Gent = Ghent University [UGENT]
hal.structure.identifierCenter for Plant Systems Biology [PSB Center]
dc.contributor.authorDE CLERCQ, Rebecca
hal.structure.identifierVIVES University of Applied Sciences
dc.contributor.authorVAN DOORSSELAERE, Jan
hal.structure.identifierUniversiteit Gent = Ghent University [UGENT]
hal.structure.identifierCenter for Plant Systems Biology [PSB Center]
dc.contributor.authorINZÉ, Dirk
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorGONZALEZ, Nathalie
hal.structure.identifierUniversiteit Gent = Ghent University [UGENT]
hal.structure.identifierCenter for Plant Systems Biology [PSB Center]
dc.contributor.authorGOOSSENS, Alain
hal.structure.identifierUniversiteit Gent = Ghent University [UGENT]
hal.structure.identifierCenter for Plant Systems Biology [PSB Center]
dc.contributor.authorPAUWELS, Laurens
dc.date.issued2022-01-01
dc.identifier.issn0032-0889
dc.description.abstractEnAbstract Plant organ size and shape are major agronomic traits that depend on cell division and expansion, which are both regulated by complex gene networks. In several eudicot species belonging to the rosid clade, organ growth is controlled by a repressor complex consisting of PEAPOD (PPD) and KINASE-INDUCIBLE DOMAIN INTERACTING (KIX) proteins. The role of these proteins in asterids, which together with the rosids constitute most of the core eudicot species, is unknown. We used Clustered Regularly Interspaced Short Palindromic Repeats–CRISPR-associated protein 9 genome editing to target SlKIX8 and SlKIX9 in the asterid model species tomato (Solanum lycopersicum) and analyzed loss-of-function phenotypes. Loss-of-function of SlKIX8 and SlKIX9 led to the production of enlarged, dome-shaped leaves and these leaves exhibited increased expression of putative Solanum lycopersicum PPD (SlPPD target genes. Unexpectedly, kix8 kix9 mutants carried enlarged fruits with increased pericarp thickness due to cell expansion. At the molecular level, protein interaction assays indicated that SlKIX8 and SlKIX9 act as adaptors between the SlPPD and SlTOPLESS co-repressor proteins. Our results show that KIX8 and KIX9 are regulators of organ growth in asterids and can be used in strategies to improve important traits in produce such as thickness of the fruit flesh.
dc.language.isoen
dc.publisherOxford University Press ; American Society of Plant Biologists
dc.subjecttomate
dc.subjectfruit charnu
dc.subjectdéveloppement du fruit
dc.subjectdivision cellulaire
dc.subjectexpansion cellulaire
dc.subjectdéveloppement foliaire
dc.subjectFeuille
dc.subjectgene
dc.subject.enSolanum lycopersicum
dc.title.enSlKIX8 and SlKIX9 are negative regulators of leaf and fruit growth in tomato
dc.typeArticle de revue
dc.identifier.doi10.1093/plphys/kiab464
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalPlant Physiology
bordeaux.page382-396
bordeaux.volume188
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-03554304
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03554304v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Plant%20Physiology&rft.date=2022-01-01&rft.volume=188&rft.issue=1&rft.spage=382-396&rft.epage=382-396&rft.eissn=0032-0889&rft.issn=0032-0889&rft.au=SWINNEN,%20Gwen&MAUXION,%20Jean-Philippe&BAEKELANDT,%20Alexandra&DE%20CLERCQ,%20Rebecca&VAN%20DOORSSELAERE,%20Jan&rft.genre=article


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