SWINNEN, Gwen; MAUXION, Jean-Philippe; BAEKELANDT, Alexandra; DE CLERCQ, Rebecca; VAN DOORSSELAERE, Jan; INZÉ, Dirk; GONZALEZ, Nathalie; GOOSSENS, Alain; PAUWELS, Laurens

doi:10.1093/plphys/kiab464

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SWINNEN, Gwen
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Center for Plant Systems Biology [PSB Center]

MAUXION, Jean-Philippe
Biologie du fruit et pathologie [BFP]

BAEKELANDT, Alexandra
Universiteit Gent = Ghent University = Université de Gand [UGENT]
Center for Plant Systems Biology [PSB Center]

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Article de revue

Ce document a été publié dans

Plant Physiology. 2022-01-01, vol. 188, n° 1, p. 382-396

Oxford University Press ; American Society of Plant Biologists

Résumé en anglais

Abstract Plant organ size and shape are major agronomic traits that depend on cell division and expansion, which are both regulated by complex gene networks. In several eudicot species belonging to the rosid clade, organ growth is controlled by a repressor complex consisting of PEAPOD (PPD) and KINASE-INDUCIBLE DOMAIN INTERACTING (KIX) proteins. The role of these proteins in asterids, which together with the rosids constitute most of the core eudicot species, is unknown. We used Clustered Regularly Interspaced Short Palindromic Repeats–CRISPR-associated protein 9 genome editing to target SlKIX8 and SlKIX9 in the asterid model species tomato (Solanum lycopersicum) and analyzed loss-of-function phenotypes. Loss-of-function of SlKIX8 and SlKIX9 led to the production of enlarged, dome-shaped leaves and these leaves exhibited increased expression of putative Solanum lycopersicum PPD (SlPPD target genes. Unexpectedly, kix8 kix9 mutants carried enlarged fruits with increased pericarp thickness due to cell expansion. At the molecular level, protein interaction assays indicated that SlKIX8 and SlKIX9 act as adaptors between the SlPPD and SlTOPLESS co-repressor proteins. Our results show that KIX8 and KIX9 are regulators of organ growth in asterids and can be used in strategies to improve important traits in produce such as thickness of the fruit flesh.< Réduire

Mots clés

tomate

fruit charnu

développement du fruit

division cellulaire

expansion cellulaire

développement foliaire

Feuille

gene

Mots clés en anglais

Solanum lycopersicum