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The SlSHN2 transcription factor contributes to cuticle formation and epidermal patterning in tomato fruit

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBRES, Cécile
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorPETIT, Johann
hal.structure.identifierUnité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
dc.contributor.authorREYNOUD, Nicolas
hal.structure.identifierBordeaux Imaging Center [BIC]
dc.contributor.authorBROCARD, Lysiane
hal.structure.identifierUnité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
dc.contributor.authorMARION, Didier
hal.structure.identifierUnité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
dc.contributor.authorLAHAYE, Marc
hal.structure.identifierUnité de recherche sur les Biopolymères, Interactions Assemblages [BIA]
dc.contributor.authorBAKAN, Benedicte
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorROTHAN, Christophe
dc.date.issued2022
dc.identifier.issn2730-9401
dc.description.abstractEnAbstract Tomato ( Solanum lycopersicum ) is an established model for studying plant cuticle because of its thick cuticle covering and embedding the epidermal cells of the fruit. In this study, we screened an EMS mutant collection of the miniature tomato cultivar Micro-Tom for fruit cracking mutants and found a mutant displaying a glossy fruit phenotype. By using an established mapping-by-sequencing strategy, we identified the causal mutation in the SlSHN2 transcription factor that is specifically expressed in outer epidermis of growing fruit. The point mutation in the shn2 mutant introduces a K to N amino acid change in the highly conserved ‘mm’ domain of SHN proteins. The cuticle from shn2 fruit showed a ~ fivefold reduction in cutin while abundance and composition of waxes were barely affected. In addition to alterations in cuticle thickness and properties, epidermal patterning and polysaccharide composition of the cuticle were changed. RNAseq analysis further highlighted the altered expression of hundreds of genes in the fruit exocarp of shn2 , including genes associated with cuticle and cell wall formation, hormone signaling and response, and transcriptional regulation. In conclusion, we showed that a point mutation in the transcriptional regulator SlSHN2 causes major changes in fruit cuticle formation and its coordination with epidermal patterning.
dc.description.sponsorshipContributions du genome, du transcriptome et de l'épigénome à l'adaptation de lépidoptères (Ostrinia spp.) à leurs plantes hôtes - ANR-13-BSV7-0012
dc.language.isoen
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subjectFruit
dc.subjectPlante fruitière
dc.subjectTomate
dc.subjectSolanum lycopersicon
dc.subjectDéveloppement du fruit
dc.subject.enMutant
dc.subject.enSHINE
dc.subject.enCuticle
dc.subject.enEpidermis
dc.subject.enCell wall
dc.subject.enEthylene
dc.title.enThe SlSHN2 transcription factor contributes to cuticle formation and epidermal patterning in tomato fruit
dc.typeArticle de revue
dc.identifier.doi10.1186/s43897-022-00035-y
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
bordeaux.journalMolecular Horticulture
bordeaux.page14
bordeaux.volume2
bordeaux.issue1
bordeaux.peerReviewedoui
hal.identifierhal-03700009
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-03700009v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Molecular%20Horticulture&rft.date=2022&rft.volume=2&rft.issue=1&rft.spage=14&rft.epage=14&rft.eissn=2730-9401&rft.issn=2730-9401&rft.au=BRES,%20C%C3%A9cile&PETIT,%20Johann&REYNOUD,%20Nicolas&BROCARD,%20Lysiane&MARION,%20Didier&rft.genre=article


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