BRES, Cécile; PETIT, Johann; REYNOUD, Nicolas; BROCARD, Lysiane; MARION, Didier; LAHAYE, Marc; BAKAN, Benedicte; ROTHAN, Christophe

doi:10.1186/s43897-022-00035-y

Métadonnées

Afficher la notice complète

Licence d’utilisation du document

BRES, Cécile
Biologie du fruit et pathologie [BFP]

PETIT, Johann
Biologie du fruit et pathologie [BFP]

REYNOUD, Nicolas
Unité de recherche sur les Biopolymères, Interactions Assemblages [BIA]

Langue

Article de revue

Ce document a été publié dans

Molecular Horticulture. 2022, vol. 2, n° 1, p. 14

Résumé en anglais

Abstract Tomato ( Solanum lycopersicum ) is an established model for studying plant cuticle because of its thick cuticle covering and embedding the epidermal cells of the fruit. In this study, we screened an EMS mutant collection of the miniature tomato cultivar Micro-Tom for fruit cracking mutants and found a mutant displaying a glossy fruit phenotype. By using an established mapping-by-sequencing strategy, we identified the causal mutation in the SlSHN2 transcription factor that is specifically expressed in outer epidermis of growing fruit. The point mutation in the shn2 mutant introduces a K to N amino acid change in the highly conserved ‘mm’ domain of SHN proteins. The cuticle from shn2 fruit showed a ~ fivefold reduction in cutin while abundance and composition of waxes were barely affected. In addition to alterations in cuticle thickness and properties, epidermal patterning and polysaccharide composition of the cuticle were changed. RNAseq analysis further highlighted the altered expression of hundreds of genes in the fruit exocarp of shn2 , including genes associated with cuticle and cell wall formation, hormone signaling and response, and transcriptional regulation. In conclusion, we showed that a point mutation in the transcriptional regulator SlSHN2 causes major changes in fruit cuticle formation and its coordination with epidermal patterning.< Réduire

Mots clés

Fruit

Plante fruitière

Tomate

Solanum lycopersicon

Développement du fruit

Mots clés en anglais

Mutant

SHINE

Cuticle

Epidermis

Cell wall

Ethylene

DOI

http://dx.doi.org/10.1186/s43897-022-00035-y

Project ANR

Contributions du genome, du transcriptome et de l'épigénome à l'adaptation de lépidoptères (Ostrinia spp.) à leurs plantes hôtes - ANR-13-BSV7-0012

Origine

Importé de hal

Unités de recherche

Biologie du Fruit & Pathologie (BFP) - UMR 1332